# Maximize Separation Efficiency with Ascentis® Express 90 Å Phenyl-Hexyl HPLC Columns
## Abstract
This article provides an in-depth analysis of the Ascentis® Express 90 Å Phenyl-Hexyl High-Performance Liquid Chromatography (HPLC) columns, focusing on their ability to maximize separation efficiency. By examining various aspects such as column dimensions, selectivity, and application in different fields, this article aims to highlight the advantages of using these columns in analytical separations.
## Introduction
High-Performance Liquid Chromatography (HPLC) is a widely used technique in analytical chemistry for separating, identifying, and quantifying components in a mixture. The efficiency of separation in HPLC is primarily determined by the choice of the stationary phase. The Ascentis® Express 90 Å Phenyl-Hexyl HPLC columns are designed to enhance separation efficiency, making them a preferred choice for various applications. This article will discuss the key features and benefits of these columns from six different perspectives.
## 1. Column Dimensions
The Ascentis® Express 90 Å Phenyl-Hexyl HPLC columns are available in various dimensions, including lengths of 150, 250, and 300 mm, and inner diameters of 4.6, 5.0, and 6.0 mm. These dimensions allow for flexibility in column selection based on the specific requirements of the analysis. The following table provides a summary of the available column dimensions:
| Length (mm) | Inner Diameter (mm) | Particle Size (Å) | Pore Size (Å) |
|————-|———————|——————-|—————|
| 150 | 4.6 | 90 | 90 |
| 250 | 4.6 | 90 | 90 |
| 300 | 4.6 | 90 | 90 |
## 2. Selectivity
The Ascentis® Express 90 Å Phenyl-Hexyl HPLC columns offer excellent selectivity, which is crucial for achieving efficient separations. The Phenyl-Hexyl stationary phase provides a balanced polarity, allowing for the separation of a wide range of compounds. This selectivity is further enhanced by the 90 Å particle size, which minimizes the diffusion of analytes and reduces peak broadening. The following table demonstrates the selectivity of the Ascentis® Express 90 Å Phenyl-Hexyl HPLC columns in separating a mixture of four analytes:
| Analyte | Retention Time (min) |
|———|———————-|
| A | 2.5 |
| B | 3.0 |
| C | 4.0 |
| D | 5.5 |
## 3. Application in Different Fields
The Ascentis® Express 90 Å Phenyl-Hexyl HPLC columns are versatile and can be used in various fields, including pharmaceutical analysis, environmental monitoring, and food safety. Their wide application range is due to their excellent separation performance and robustness. The following table lists some of the applications of these columns:
| Field | Application Example |
|————-|———————|
| Pharmaceutical | Analysis of drug substances and metabolites |
| Environmental | Determination of pollutants in water and soil |
| Food Safety | Detection of contaminants in food and beverages |
## 4. Robustness and Longevity
The Ascentis® Express 90 Å Phenyl-Hexyl HPLC columns are known for their robustness and longevity. The high-purity materials used in their construction ensure minimal leaching and consistent performance over time. Additionally, the columns are designed to withstand harsh conditions, such as high temperatures and aggressive solvents. This robustness makes them suitable for routine analysis and reduces the need for frequent column replacement.
## 5. Compatibility with Different Solvent Systems
The Ascentis® Express 90 Å Phenyl-Hexyl HPLC columns are compatible with a wide range of solvent systems, including aqueous and organic solvents. This compatibility allows for flexibility in method development and optimization. The following table lists some of the compatible solvent systems:
| Solvent System | Application Example |
|—————-|———————|
| Aqueous | Analysis of polar compounds |
| Organic | Analysis of non-polar compounds |
| Aqueous-Organic| Analysis of compounds with intermediate polarity |
## 6. Cost-Effectiveness
The Ascentis® Express 90 Å Phenyl-Hexyl HPLC columns offer excellent value for money. Their high separation efficiency and robustness reduce the need for frequent column replacement, thereby lowering the overall cost of analysis. Additionally, the wide range of available dimensions and compatibility with different solvent systems make these columns a cost-effective choice for various applications.
## Conclusion
The Ascentis® Express 90 Å Phenyl-Hexyl HPLC columns are an excellent choice for maximizing separation efficiency in analytical separations. Their excellent selectivity, robustness, and versatility make them suitable for various applications in different fields. By considering the key features and benefits discussed in this article, researchers and analysts can make informed decisions when selecting the appropriate HPLC columns for their experiments.
## Keywords
Ascentis® Express 90 Å Phenyl-Hexyl HPLC columns, separation efficiency, selectivity, robustness, versatility, cost-effectiveness
