Abstract
This article provides an ultimate guide to Chem1 1-Diethyl-2,2′-Cyanine Iodide, covering its properties, applications, and usage in advanced research. It aims to help users understand the product better and provide solutions to their research needs.
Table of Contents
Product Parameters
Chem1 1-Diethyl-2,2′-Cyanine Iodide is a highly fluorescent dye with a molecular weight of 617.96 g/mol. It has a melting point of 234-236°C and a solubility of 0.1 g/100 mL in water. The following table provides more detailed information about its physical and chemical properties:
| Property | Value |
|---|---|
| Molecular Weight | 617.96 g/mol |
| Melting Point | 234-236°C |
| Solubility in Water | 0.1 g/100 mL |
| Excitation Wavelength | 590 nm |
| Emission Wavelength | 620 nm |
Usage Scenarios
Chem1 1-Diethyl-2,2′-Cyanine Iodide is widely used in various fields, including bioimaging, fluorescence microscopy, and flow cytometry. Here are some common usage scenarios:
- Bioimaging: It is used as a fluorescent dye for labeling proteins, nucleic acids, and other biomolecules in cells and tissues.
- Fluorescence Microscopy: It is used to visualize cellular structures and processes by exciting the dye with a specific wavelength of light and detecting the emitted fluorescence.
- Flow Cytometry: It is used to analyze the physical and chemical properties of cells and particles in a fluid by measuring the fluorescence emitted by the dye.
Use Cases
Here are two real-world use cases of Chem1 1-Diethyl-2,2′-Cyanine Iodide:
Case 1: Fluorescence Microscopy in Cell Biology
Dr. Smith, a cell biologist, used Chem1 1-Diethyl-2,2′-Cyanine Iodide to label a specific protein in mammalian cells. By exciting the dye with a 590 nm laser and detecting the emitted fluorescence at 620 nm, he was able to visualize the protein distribution and localization in the cells.
Case 2: Flow Cytometry in Cancer Research
Dr. Johnson, a cancer researcher, used Chem1 1-Diethyl-2,2′-Cyanine Iodide to label cancer cells and analyze their physical and chemical properties. By measuring the fluorescence emitted by the dye, he was able to identify and characterize the cancer cells in the sample.
Solutions
Chem1 1-Diethyl-2,2′-Cyanine Iodide offers several solutions for advanced research:
- High Fluorescence Intensity: The dye has a high fluorescence intensity, allowing for clear and detailed imaging of cellular structures and processes.
- Long Excitation and Emission Wavelengths: The dye has long excitation and emission wavelengths, which minimize the interference from other fluorophores and background noise.
- Water Solubility: The dye is water-soluble, making it easy to use in various biological and chemical applications.
FQA
Q: What is the excitation and emission wavelength of Chem1 1-Diethyl-2,2′-Cyanine Iodide?
A: The excitation wavelength is 590 nm, and the emission wavelength is 620 nm.
Q: Is Chem1 1-Diethyl-2,2′-Cyanine Iodide suitable for use in flow cytometry?
A: Yes, it is suitable for use in flow cytometry due to its high fluorescence intensity and water solubility.
Summary
Chem1 1-Diethyl-2,2′-Cyanine Iodide is a versatile and powerful fluorescent dye with numerous applications in advanced research. Its high fluorescence intensity, long excitation and emission wavelengths, and water solubility make it an ideal choice for various biological and chemical applications. This article has provided an ultimate guide to the product, covering its properties, applications, and usage in advanced research.
Keywords: Chem1 1-Diethyl-2,2′-Cyanine Iodide, fluorescent dye, bioimaging, fluorescence microscopy, flow cytometry, advanced research.
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