1. Product Specifications & Comparative Analysis

2. Primary Applications in Biomedical Research

Core Functionalities:

• Immunohistochemistry (IHC): Superior chromogenic substrate for HRP-conjugated antibodies with 40% higher contrast compared to DAB
• ELISA Development: Linear detection range from 0.05-10 ng/ml across multiple cytokine assays
• Lateral Flow Assays: Preferred substrate for rapid diagnostic tests requiring <5 minute visualization

3. Implementation Protocols

Standard Working Solution Preparation:

1. Prepare 0.1M citrate-phosphate buffer (pH 4.3)
2. Dissolve TMB Monosulfate to 0.4 mg/ml concentration
3. Add 0.006% H₂O₂ immediately before use
4. Incubate at 37°C for 8-12 minutes for optimal signal development

4. Case Studies & Industry Applications

COVID-19 IgM/IgG Rapid Test Kit Development:

• Detection threshold: 98.7% sensitivity at 15 pg/ml
• Signal stability: 72 hours post-development
• Clinical validation: N=1,200 samples across 3 medical centers

5. Client Success Stories

Shanghai Institute of Immunology:

• Application: Multiplex cytokine profiling in tumor microenvironment
• Result: 3-fold increase in low-abundance IL-10 detection vs. previous methods
• Throughput: Processed 384 samples/day using automated platform

Wuxi CDMO Facility:

• Implementation: Large-scale IVD kit production
• Outcome: Achieved ISO 13485 certification with 99.2% batch consistency
• Scale: 50,000 test kits/month capacity

6. Technical Consultation

Contact our application scientists for protocol optimization and bulk pricing:

Email: info@vivalr.com
Tel: (86) 15866781826

Response time: <1 business day for technical queries