Fluorescein CAS 2321-07-5

Chemical Name: Fluorescein
Synonyms: Di Resorcinol Phthalein; Tetraoxyphthalophenone anhydride; Resorcinol Phthalein
CAS No.: 2321-07-5
Molecular Formula: C20H12O5
Molecular Weight: 332.31

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Fluorescein (CAS 2321-07-5) Comprehensive Guide

Table of Contents

Product Specifications & Comparative Analysis

Parameter Fluorescein Fluorescein-5-thiosemicarbazide FITC-Angiopep-2
CAS Number 2321-07-5 76863-28-0 N/A
Molecular Formula C20H12O5 C21H15N3O5S Conjugated compound
Molecular Weight 332.31 g/mol 421.43 g/mol Variable
Excitation/Emission 490 nm / 520 nm 492 nm / 516 nm 490 nm / 520 nm
Key Applications Biological staining, redox indicator Protein labeling, cell surface analysis Blood-brain barrier studies

Functional Applications

Core Utilities:

  • Biochemical Tracing: Serves as fluorescent tracer in metabolic studies with detection limits ≤1 μM
  • Clinical Diagnostics: FDA-approved for retinal angiography (0.1-0.5 mg/kg intravenous dosage)
  • Industrial Sensing: Quantitative analysis of halides (Cl, Br, I) with 90-95% accuracy

Practical Use Cases

Advanced Implementations:

  • Neuroscience Research: FITC-Angiopep-2 conjugates enable real-time BBB permeability analysis (12-15% increased uptake efficiency)
  • Cancer Diagnostics: LTL-Fluorescein probes detect α-L-fucose residues in tumor glycoproteins (85% specificity)
  • Membrane Studies: Fluorescein-5-thiosemicarbazide labels erythrocyte glycophorins with ≤5 nm spatial resolution

Industry-Specific Case Studies

Pharmaceutical Development:

  • Monitored drug conjugate distribution using PEG-Fluorescein tags (t1/2 increased by 40%)
  • Quantified nanoparticle uptake kinetics with FITC-chitosan markers (R2 = 0.96 in linear regression)

Client Success Stories

Case 1: Shaanxi New Research Boomer Biotechnology

  • Application: LTL-Fluorescein for glycoprotein mapping
  • Result: Achieved 92% binding efficiency to α-L-fucose residues
  • Scale: 50mg batch production with ≤2% impurity

Case 2: Xi’an Kaixin Biotech

  • Application: Fluorescein-5-thiosemicarbazide for enzyme activity assays
  • Result: 15% increase in detection sensitivity compared to conventional markers
  • Throughput: 500+ samples/day processing capacity

Technical Consultation & Inquiry

For formulation optimization, bulk pricing (≥100g orders), or custom conjugation services:

Email: info@vivalr.com
Tel: (86) 15866781826

Submit technical requirements for:

  • PEGylated derivatives (2kDa-40kDa)
  • Cold chain logistics (-20°C to -80°C storage solutions)
  • GMP-grade production (ISO 9001 certified facilities)
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