CHAPS (CAS 75621-03-3): Technical Guide for Protein Research Applications
Table of Contents
1. Product Overview & Specifications
| Parameter | CHAPS (Ultra Pure Grade) | Alternative Detergents |
|---|---|---|
| CAS Number | 75621-03-3 | Varies by product |
| Molecular Formula | C32H58N2O7S | Depends on type |
| Molecular Weight | 614.88 g/mol | 300-800 g/mol |
| Critical Micelle Concentration | 6-10 mM | 0.1-15 mM |
| Solubility | Water (100 mM), DMSO, methanol | Variable |
| Storage | Room temperature (1-year stability) | Often requires refrigeration |
| Key Advantage | UV-transparent, maintains protein activity | May denature proteins |
2. Key Applications in Life Sciences
Core Functions:
- Membrane protein solubilization with preserved activity
- Native state protein stabilization for structural studies
- Buffer component for 2D electrophoresis (0.5-2% concentration)
- Cell lysis reagent for subcellular fractionation
- Detergent in chromatography (IEX, SEC)
Specialized Uses:
- DNA-protein complex stabilization (0.5% working concentration)
- Enzyme characterization buffers
- Lipid raft studies
3. Step-by-Step Usage Protocols
Protocol 1: Membrane Protein Extraction
- Prepare 20 mM CHAPS in Tris-HCl buffer (pH 7.4)
- Homogenize tissue/cells at 4°C (1:10 w/v ratio)
- Centrifuge at 100,000g for 45 minutes
- Collect supernatant for downstream analysis
Protocol 2: 2D Electrophoresis Sample Prep
- Mix 4% CHAPS with 8M urea, 2M thiourea
- Add 50 mM DTT and 0.5% IPG buffer
- Vortex for 30 minutes at room temperature
- Centrifuge before loading
4. Industry Case Studies
Case 1: Wuhan Difu Biotechnology
Application: Plant membrane protein isolation
Result: 92% activity retention vs 78% with SDS
Feedback: “Superior resolution in native PAGE”
Case 2: Yeasen Biotech
Application: Diagnostic antibody production
Result: 40% increase in antigen recovery
Feedback: “Critical for preserving epitope integrity”
Case 3: Hubei Shuangyan Chemical
Application: Industrial enzyme purification
Result: 99% purity achieved
Feedback: “Consistent batch-to-batch performance”
5. Contact for Technical Consultation
For protocol customization or bulk orders:
Email: info@vivalr.com
Tel: (86) 15866781826
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