Proteinase K (CAS 39450-01-6): Comprehensive User Guide
Table of Contents
1. Product Overview & Specifications
Proteinase K (CAS 39450-01-6) is a broad-spectrum serine protease widely used in molecular biology for digesting proteins and eliminating nucleases. Key parameters include:
| Parameter | Specification |
|---|---|
| CAS Number | 39450-01-6 |
| Molecular Weight | 28,900 Da |
| Activity | ≥40 U/mg |
| Optimal pH | 7.5-12.0 |
| Optimal Temperature | 37-65°C |
| Storage | -20°C (lyophilized) |
Comparative Analysis
| Supplier | Purity | Activity (U/mg) | Price per 100mg |
|---|---|---|---|
| Supplier A | ≥98% | 40 | $120 |
| Supplier B | ≥95% | 35 | $95 |
| Our Product | ≥99% | 45 | $110 |
2. Key Applications of Proteinase K
- Nucleic Acid Purification: Digests histones and RNases/DNases during DNA/RNA extraction.
- Tissue Digestion: Isolates cellular components from hard/soft tissues.
- Prion Deactivation: Degrades infectious prion proteins in medical devices.
- Western Blotting: Antigen retrieval for antibody binding.
3. Usage Guidelines & Best Practices
Recommended Protocols
| Application | Concentration | Incubation Time |
|---|---|---|
| DNA Extraction | 0.1-1 mg/ml | 30-120 mins |
| Prion Deactivation | 2-5 mg/ml | 60-180 mins |
Note: Inactivate at 95°C for 10 mins post-digestion.
4. Case Studies & Real-World Applications
Case Study 1: High-Yield Plasmid DNA Isolation
A genomics lab achieved 30% higher plasmid yields using 0.8 mg/ml Proteinase K at 55°C for 90 mins.
5. Client Success Stories
Biotech Corp (Shanghai)
“Reduced RNA degradation by 75% in FFPE samples using optimized Proteinase K protocols.”
Diagnostics Lab Ltd (Beijing)
“Achieved 99.9% prion elimination in surgical tools through 3-hour digestion cycles.”
6. Request a Quote or Sample
Contact our technical team for bulk pricing or protocol optimization:
Email: info@vivalr.com
Tel: (86) 15866781826
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