Tris(2-carboxyethyl)phosphine Hydrochloride Cas 51805-45-9
Assay: 98.0%
Appearance: White crystalline powder
Package: 1kg per drum, foil bag
Sample: Available
related documents:
COA of TRIS(2-CARBOXYETHYL)PHOSPHINE HYDROCHLORIDE 51805-45-9
TDS of TCEP 51805-45-9
MSDS of TRIS(2-CARBOXYETHYL)PHOSPHINE HYDROCHLORIDE 51805-45-9
MSDS-Tristyrylphenol ethoxylates
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Complete User Guide for Tris(2-carboxyethyl)phosphine Hydrochloride (TCEP HCl, CAS 51805-45-9)
Table of Contents
1. Product Overview & Specifications
Tris(2-carboxyethyl)phosphine Hydrochloride (TCEP HCl) (CAS 51805-45-9) is a water-soluble, odorless reducing agent widely used for disulfide bond reduction in biochemical research.
| Parameter | TCEP HCl | DTT | β-Mercaptoethanol |
|---|---|---|---|
| Molecular Weight | 286.65 g/mol | 154.25 g/mol | 78.13 g/mol |
| Stability in Buffer | >24 hours (pH 3-9) | 4-6 hours | 1-2 hours |
| Reduction Efficiency | High (1:1 stoichiometry) | Moderate | Low |
2. Key Applications & Use Cases
Core Applications:
- Protein structure analysis (disulfide bond cleavage)
- Nucleic acid research (prevent RNA/DNA degradation)
- Mass spectrometry sample preparation
- Antibody-drug conjugate development
Advantages Over Alternatives:
- Non-volatile and non-thiolated byproducts
- Effective at lower concentrations (typical 5-50mM)
- Compatible with MALDI-TOF analysis
3. Step-by-Step Usage Protocols
Example Protocol: Protein Reduction for Western Blot
- Prepare 100mM stock solution in deionized water
- Add 5-10mM TCEP HCl to protein lysate
- Incubate at 37°C for 30 minutes
- Alkylate with iodoacetamide (optional)
- Proceed with electrophoresis
4. Real-World Customer Case Studies
Case Study 1: PharmaTech Inc. (Antibody Engineering)
- Challenge: Maintaining monoclonal antibody stability during conjugation
- Solution: 20mM TCEP HCl in PBS buffer (pH 7.4)
- Result: 92% activity retention vs. 68% with DTT
Case Study 2: BioResearch Labs (Proteomics)
- Challenge: Cysteine-rich protein analysis
- Solution: On-column reduction with 50mM TCEP HCl
- Result: Identified 15% more phosphorylation sites
5. Technical Support & Inquiry
For bulk pricing, technical specifications, or custom formulations:
- Email: info@vivalr.com
- Phone: (86) 15866781826
Request free sample or MSDS: Contact our experts
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