HEPPS Buffer (CAS 16052-06-5): Comprehensive User Guide
Table of Contents
1. Product Overview & Specifications
HEPPS (3-[4-(2-Hydroxyethyl)-1-piperazinyl]propanesulfonic acid), CAS 16052-06-5, is a zwitterionic biological buffer widely used in biochemical and molecular biology applications due to its stable pH range (7.8–8.8) and low metal ion binding.
Key Specifications
| Parameter | Value |
|---|---|
| CAS Number | 16052-06-5 |
| Molecular Formula | C9H20N2O4S |
| Molecular Weight | 252.33 g/mol |
| Appearance | White crystalline powder |
| Purity | ≥99% (HPLC) |
| Solubility | Water (>100 mg/mL) |
| Storage | 2–8°C, dry conditions |
Comparative Analysis
| Buffer | pH Range | Advantages |
|---|---|---|
| HEPPS | 7.8–8.8 | Low toxicity, minimal UV absorption |
| HEPES | 7.2–8.2 | Wider temperature stability |
| Tris | 7.0–9.0 | Cost-effective but metal-sensitive |
2. Applications of HEPPS Buffer
Biochemistry & Molecular Biology
- Cell culture media for mammalian and insect cells
- Protein purification and crystallization
- PCR and electrophoresis
Pharmaceuticals
- Drug formulation stability testing
- Enzyme activity assays
Industrial Uses
- Diagnostic reagent preparation
- Cosmetic formulations
3. Usage Guidelines
Preparation Steps
- Dissolve 23.8 g HEPPS in 800 mL deionized water.
- Adjust pH to 8.0 with NaOH/HCl.
- Bring volume to 1 L and filter-sterilize.
Best Practices
- Use fresh solutions for sensitive applications
- Avoid repeated freeze-thaw cycles
4. Case Studies
Lab Use: Mammalian Cell Culture
A research team achieved 95% cell viability using 25 mM HEPPS in DMEM media at pH 8.0 for hybridoma cell lines.
Industrial Use: Diagnostic Kits
HEPPS demonstrated superior stability in lateral flow assays compared to phosphate buffers during accelerated aging tests.
5. Customer Success Stories
BioGen Innovations (Shanghai)
Challenge: Protein aggregation during purification
Solution: 50 mM HEPPS buffer at pH 8.5
Result: 40% increase in soluble protein yield
PharmaSolutions Inc. (Boston)
Challenge: Drug formulation pH drift
Solution: HEPPS-based stabilization
Result: 12-month shelf-life stability achieved
6. Request a Quote
Contact our technical team for customized buffer solutions:
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