Table of Contents
- 3,3′-Diaminobenzidine (CAS 91-95-2) Overview
- Technical Specifications & Comparative Analysis
- Key Applications in Life Sciences
- Optimized Usage Protocols
- Industry Implementation Cases
- Technical Purchasing Guidance
- Professional Consultation
3,3′-Diaminobenzidine (CAS 91-95-2) Overview
Fundamental Characteristics
| Parameter | Specification |
|---|---|
| CAS Number | 91-95-2 |
| Molecular Formula | C₁₂H₁₄N₄ |
| Molecular Weight | 214.27 g/mol |
| Appearance | Light brown to dark brown crystalline powder |
| Solubility | Soluble in DMSO, slightly soluble in water |
| Storage Conditions | 2-8°C in amber glass containers |
Performance Comparison Matrix
| Feature | 3,3′-DAB | TMB | AEC |
|---|---|---|---|
| Sensitivity | High | Medium | Low |
| Reaction Product | Insoluble precipitate | Soluble | Soluble |
| Microscopy Compatibility | Superior | Limited | Moderate |
| Carcinogenicity | Class 2B | Non-carcinogenic | Non-carcinogenic |
Core Scientific Applications
Immunohistochemistry (IHC)
As peroxidase substrate for antigen localization with permanent staining capability
Electron Microscopy
Ultrastructural demonstration of enzymatic activity in cellular compartments
Western Blotting
Membrane-based protein detection with high spatial resolution
Advanced Implementation Protocol
Solution Preparation
| Component | Concentration | Volume |
|---|---|---|
| DAB Stock | 5 mg/mL | 1 mL |
| Buffer Solution | 0.05M Tris-HCl | 9 mL |
| H₂O₂ | 0.03% | 10 μL |
Safety Protocols
- Mandatory PPE: Nitrile gloves, chemical goggles
- Ventilation: Class II BSC required
- Spill Management: Absorb with vermiculite
Documented Implementation Cases
Case 1: Oncology Research Center
| Parameter | Detail |
|---|---|
| Application | HER2/neu biomarker detection in breast carcinoma |
| Solution | Optimized DAB incubation (7.5 mins) |
| Outcome | 98% staining consistency across 500+ samples |
Case 2: Diagnostic Kit Manufacturer
| Parameter | Detail |
|---|---|
| Challenge | Rapid HIV p24 antigen detection |
| Innovation | DAB/NiCl₂ enhancement system |
| Result | Detection limit: 0.1 pg/mL |
Technical Purchasing Considerations
| Factor | Technical Requirement |
|---|---|
| Purity | ≥99% (HPLC-verified) |
| Batch Consistency | UV-Vis spectrum deviation <2% |
| Packaging | Nitrogen-sealed ampoules |
Expert Technical Consultation
Contact our chromatography specialists:
Email: info@vivalr.com
Mobile: (86) 15866781826
Technical Inquiry Checklist
- Required purity level
- Application-specific formulation needs
- GMP compliance requirements
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