2-(N-Morpholino)ethanesulfonic Acid Hemisodium Salt (CAS 117961-21-4): Comprehensive Product Guide
Table of Contents
- 1. Product Specifications & Comparative Analysis
- 2. Key Applications & Functional Advantages
- 3. Usage Scenarios in Life Sciences
- 4. Industry Case Studies
- 5. Technical Support & Ordering Information
1. Product Specifications & Comparative Analysis
| Parameter | Specification |
|---|---|
| CAS Number | 117961-21-4 |
| Molecular Formula | C6H12NNaO4S · 0.5H2O |
| Molecular Weight | 231.22 g/mol |
| Appearance | White crystalline powder |
| Purity | ≥99% (HPLC) |
| pH Range | 5.5 – 6.7 (25°C, 0.1M solution) |
Comparative Buffer Performance
| Buffer Type | pH Range | Membrane Penetration | Metal Chelation |
|---|---|---|---|
| MES Hemisodium | 5.5-6.7 | Low | Minimal |
| HEPES | 6.8-8.2 | Moderate | Significant |
| Tris-HCl | 7.0-9.0 | High | Moderate |
2. Key Applications & Functional Advantages
Primary Usage Areas:
- Mammalian cell culture media supplementation
- Enzyme kinetics studies (pH-sensitive reactions)
- Protein crystallization buffers
- Electrophoresis running buffers
- Diagnostic reagent formulations
Technical Benefits:
- Superior buffering capacity at physiological osmolarity
- Non-interfering with metal-catalyzed reactions
- Low UV absorbance for spectrophotometric assays
- Reduced cellular toxicity in long-term cultures
3. Usage Scenarios in Life Sciences
Protocol Example: Cell Culture Application
- Prepare 0.5M stock solution in ultrapure water
- Filter-sterilize through 0.22μm membrane
- Add 10ml/L to basal media (final 10mM concentration)
- Adjust pH with NaOH/HCl if required
- Validate osmolality (target 290-310 mOsm/kg)
4. Industry Case Studies
Case 1: Biopharmaceutical Production Optimization
Client: BioResearch Labs (Shanghai)
Challenge: Reduce antibody aggregation in CHO cell cultures
Solution: 15mM MES hemisodium in fed-batch media
Outcome: 22% increase in monomeric antibody yield
Case 2: Diagnostic Assay Development
Client: Diagnostic Specialists Inc.
Challenge: Stabilize lactate dehydrogenase in test kits
Solution: 50mM MES buffer with 0.1% BSA
Outcome: 6-month shelf-life stability achieved
5. Technical Support & Ordering Information
For bulk pricing, COA requests, or formulation assistance:
Email: info@vivalr.com
Phone: (86) 15866781826
Order Specifications:
- Standard packaging: 100g, 500g, 1kg
- Custom concentrations available
- GMP-grade batches on request
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