Thiazolyl Blue CAS 298-93-1
Chemical Name: Thiazolyl Blue
Synonyms: 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide; Methylthiazolyldiphenyl-tetrazolium bromide; Thiazolyl Blue Tetrazolium Bromide; MTT
CAS No.: 298-93-1
Molecular Formula: C18H16BrN5S
Molecular Weight: 414.32
Appearance: Powder
Thiazolyl Blue (CAS 298-93-1) Comprehensive Product Guide 2025
Table of Contents
- 1. Product Specifications & Comparative Analysis
- 2. Detailed Applications in Biomedical Research
- 3. Operational Protocols & Best Practices
- 4. Industry Application Case Studies
- 5. Client Success Stories
- 6. Professional Consultation & Quotation
1. Product Specifications & Comparative Analysis
| Parameter | Thiazolyl Blue (MTT) | XTT Assay | WST-1 |
|---|---|---|---|
| CAS Number | 298-93-1 | 111072-31-2 | 129830-38-2 |
| Molecular Formula | C18H16BrN5S | C18H13N4NaO13S3 | C22H17N4NaO14S3 |
| Detection Sensitivity | 5×103 cells/well | 1×104 cells/well | 2×103 cells/well |
| Solubility Requirement | DMSO required | Aqueous solution | Direct measurement |
2. Detailed Applications in Biomedical Research
Core Applications:
- Cell proliferation quantification in cancer research
- Cytotoxicity assessment for drug development
- Bioactivity screening of natural compounds
- Nanomaterial safety evaluation
Technical Advantages:
- 94.7% correlation with ATP-based assays
- Stable formazan crystals for reliable spectrophotometric measurement
- Compatibility with 96-well/384-well microplates
3. Operational Protocols & Best Practices
Standard Protocol:
- Prepare 5 mg/mL stock solution in PBS
- Add 20μL reagent per 100μL culture medium
- Incubate 3-4 hours at 37°C with 5% CO2
- Add solubilization solution (10% SDS in 0.01M HCl)
- Measure absorbance at 570nm with 630nm reference
4. Industry Application Case Studies
Pharmaceutical Development:
Used in Phase II trials of novel kinase inhibitors (2023 data):
- IC50 determination accuracy: ±5% vs flow cytometry
- Throughput: 200 compounds/day screening capacity
5. Client Success Stories
Case 1: Shanghai BioPharm Co., Ltd.
- Application: High-throughput screening of TCM extracts
- Outcome: Identified 3 lead compounds with 80% cytotoxicity selectivity
- Throughput improvement: 300% vs previous Alamar Blue method
Case 2: National Institute of Nanotechnology
- Application: Graphene oxide safety profiling
- Results: Established 50μg/mL as safe exposure threshold
- Validation: 98% correlation with LDH release assay
6. Professional Consultation & Quotation
Contact our technical experts for:
- Bulk pricing (GMP-grade available)
- Custom formulation services
- Assay development support
Email: info@vivalr.com
Tel: (86) 15866781826
相关产品
Chemical Name: alpha-Hexylcinnamaldehyde
CAS No.: 101-86-0
Molecular Formula: C15H20O
Molecular Weight: 216.32
Appearance: Yellow Liquid
Chemical Name: Potassium Castorate
CAS No.: 8013-05-6
Molecular Formula: C57H107K3O12
Molecular Weight: 1101.74718
Appearance: Yellow Liquid
Chemical Name: o-Xylene
Synonyms: 1,2-Dimethylbenzene; ortho-xylene
CAS No.: 95-47-6
Molecular Formula: C8H10
Molecular Weight: 106.17
Chemical Name: Tribehenin
Cas No.: 18641-57-1
Molecular Fomula: C69H134O6
Molecular Weight: 1059.8
Appearance: White waxy powder or solid
Chemical Name: Isoflavone
Synonyms: Soy Isoflavone; Soybean Lsoflavones; 3-Phenylchromen-4-one; 3-Phenylchromone
CAS No.: 574-12-9
Molecular Formula: C15H10O2
Molecular Weight: 222.24
Appearance: Powder
Chemical Name: Ammonium Iron(II) Sulfate
Synonyms: Diammonium iron bis(sulphate); iron (ii) ammonium sulfate
CAS No.: 10045-89-3
Molecular Formula: FeH5NO4S
Molecular Weight: 170.95
Chemical Name: 1,1,2,2-Tetrachloroethane
Other Name: Tetrachlorethane
CAS No.: 79-34-5
Molecular Formula: C2H2Cl4
Molecular Weight: 167.85
Appearance: Liquid
Chemical Name: 1-[9-ethyl-6-(4-fluoro-benzoyl)-9H-carbazol-3-yl]-ethanone
Cas No.: 876907-97-0
Molecular Fomula: C23H18FNO2
Molecular Weight: 359.39
Chemical Name: 3-Hydroxybutyric acid
CAS No.: 625-71-8
Molecular Formula: C4H8O3
Molecular Weight: 104.1
Appearance: White powder
Chemical Name: Marbofloxacin
CAS No.: 115550-35-1
Molecular Formula: C17H19FN4O4
Molecular Weight: 362.36
Appearance: Red-Brown Crystal
Chemical Name: 1,5-Diphenylcarbazide
Synonyms: 1,5-Diphenylcarbohydrazide; 2,2’-diphenylcarbazide; Diphenylcartazide; sym-diphenylcarbazide
CAS No.: 140-22-7
Molecular Formula: C13H14N4O
Molecular Weight: 242.28
评论
目前还没有评论。