Spermine CAS 71-44-3

Chemical Name: Spermine
CAS No.: 71-44-3
Molecular Formula: C10H26N4
Molecular Weight: 202.34

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Comprehensive Guide to Spermine (CAS 71-44-3)

Table of Contents

1. Product Overview & Specifications

Spermine (CAS 71-44-3) is a naturally occurring polyamine critical for cellular metabolism and growth. Below are key specifications:

Parameter Value
CAS Number 71-44-3
Molecular Formula C₁₀H₂₆N₄
Molecular Weight 202.34 g/mol
Appearance White crystalline powder
Purity ≥98% (HPLC)
Solubility Water, ethanol, acidic solutions

Comparative Analysis with Similar Compounds

Compound Function Key Advantage of Spermine
Spermidine Cell proliferation Superior DNA stabilization
Putrescine Basic metabolic processes Enhanced antioxidant properties

2. Applications of Spermine

Biomedical Research

  • DNA/RNA precipitation in molecular biology protocols.
  • Apoptosis regulation studies in cancer research.

Pharmaceuticals

  • Intermediate in antiviral/antitumor drug synthesis.
  • Enhancer for drug delivery systems.

Agriculture & Industrial

  • Plant growth regulator for stress resistance.
  • Additive in biopolymer production.

3. Usage Guidelines

Recommended Protocols

  • DNA Precipitation: Use 5-10 mM final concentration in buffers.
  • Cell Culture: Optimize at 1-5 µM to avoid cytotoxicity.

Safety & Handling

  • Store at 2-8°C in airtight containers.
  • Use PPE (gloves, goggles) to prevent irritation.

4. Case Studies

Case 1: Enhancing PCR Efficiency

A biotech firm achieved 30% higher PCR yield by adding 7 mM spermine to reduce DNA degradation.

Case 2: Drug Formulation Stability

Pharma Company X extended siRNA shelf-life by 6 months using spermine-based stabilization.

5. Client Success Stories

Client A: Gene Therapy Startup

  • Challenge: Low viral vector production efficiency.
  • Solution: 5 µM spermine in HEK293 cell culture.
  • Result: 2x higher titer with 90% viability.

Client B: Agrochemical Leader

  • Challenge: Drought-sensitive crop yields.
  • Solution: Foliar spray with 0.1% spermine.
  • Result: 40% higher biomass under water stress.

6. Request a Quote

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