Sephadex LH20 cas 9041-37-6

Comprehensive Guide to Sephadex LH-20 (CAS 9041-37-6)

Table of Contents

• Product Specifications & Comparative Analysis
• Applications & Functional Overview
• Operational Guidelines & Case Studies
• Client Success Stories
• Inquiry & Procurement Support

1. Product Specifications & Comparative Analysis

2. Applications & Functional Overview

Separation Mechanism: Combines gel filtration (molecular size exclusion) with reverse-phase partition chromatography in organic solvents.

Key Applications:

• Purification of lipophilic natural products (e.g., terpenoids, flavonoids)
• Fractionation of synthetic organic compounds
• Desalting and buffer exchange in non-aqueous systems
• Pharmaceutical intermediate purification

3. Operational Guidelines & Case Studies

Protocol for Column Preparation:

1. Swelling: Hydrate in methanol/water (7:3) for ≥3 hours at 25°C
2. Column Loading: Maintain flow rate ≤10 cm/h during packing
3. Sample Load: 1-2% bed volume for critical separations

Case Example: Flavonoid Isolation

Objective: Separate quercetin derivatives from citrus extract

Parameters:

• Column: 100 cm × 5 cm (1.96 L bed volume)
• Eluent: Methanol-water gradient (70% → 100% methanol)
• Yield: 92% pure quercetin-3-O-rutinoside

4. Client Success Stories

Case 1: Natural Product Research Institute

Challenge: Purification of labile sesquiterpenes from Artemisia annua

Solution:

• Used Sephadex LH-20 in chloroform-methanol system
• Achieved 98% purity with 85% recovery rate

Case 2: Pharmaceutical Manufacturer

Requirement: Scale-up purification of synthetic API intermediates

Outcome:

• Processed 5 kg batches with ≤2% impurity carryover
• Reduced solvent consumption by 40% vs. traditional methods

5. Inquiry & Procurement Support

For technical specifications, bulk pricing, or application support:

• Email: info@vivalr.com
• Phone: (86) 15866781826

Request complimentary sample kits or schedule a chromatography consultation today.