1. Product Overview & Specifications

9-Fluorenylmethyl Chloroformate (Fmoc-Cl) is a critical reagent in peptide synthesis and analytical chemistry. Key parameters include:

Comparison with Other Protecting Agents

2. Key Applications

• Peptide Synthesis: Protects amino groups during solid-phase peptide synthesis (SPPS) to create N-Fmoc amino acids.
• Analytical Chemistry: Derivatizes amino acids for enhanced HPLC and capillary electrophoresis detection sensitivity.
• Oligonucleotide Synthesis: Serves as a temporary protecting group in phosphoramidite-based DNA/RNA synthesis.
• Pharmaceutical Research: Synthesizes bicyclic proline analogs and other bioactive molecules.

3. Usage Scenarios

Example 1: Fmoc Protection in Peptide Synthesis

Step 1: Dissolve 1 eq. of amino acid in anhydrous DMF.
Step 2: Add 1.2 eq. Fmoc-Cl and 2 eq. NaHCO₃.
Step 3: Stir at 25°C for 4 hours. Monitor by TLC.
Step 4: Purify via precipitation or column chromatography.

Example 2: Amino Acid Derivatization for HPLC

Mix 10 µL sample with 20 µL Fmoc-Cl (1% in acetonitrile) and 5 µL borate buffer (pH 8.5). Incubate at 40°C for 15 min. Analyze using a C18 column with UV detection at 265 nm.

4. Customer Case Studies

Case 1: PharmaSynth Co., Ltd.

Challenge: Low yield in SPPS due to incomplete amino protection.
Solution: Switched to Fmoc-Cl (98% purity) with optimized reaction conditions.
Result: 22% increase in peptide yield and reduced side products.

Case 2: BioAnalytix Research Institute

Challenge: Poor amino acid detection limits in urine samples.
Solution: Implemented Fmoc-Cl derivatization for LC-MS analysis.
Result: Achieved 0.1 ng/mL sensitivity for glutamine quantification.

5. Contact for Inquiries

For pricing, bulk orders, or technical support:
Email: info@vivalr.com
Tel: (86) 15866781826

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