4-morpholineethanesulfonic acid cas 4432-31-9

Chemical Name:4-Morpholineethanesulfonic acid
CAS No.: 4432-31-9
Molecular Fomula: C12H22O11
Molecular weight:195.24
Appearance:White crystalline powder
Assay:99%min

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Table of Contents

4-Morpholineethanesulfonic Acid (CAS 4432-31-9): Product Information

Basic Specifications

Parameter Details
CAS Number 4432-31-9
Molecular Formula C6H13NO4S
Molecular Weight 219.24 g/mol
Appearance White crystalline powder
Solubility Water-soluble (1M solution at 25°C)
pH Range 5.5 – 6.7
Storage 2-8°C in dry conditions

Comparative Analysis with Similar Buffers

Buffer pH Range Temperature Stability Metal Chelation
MES 5.5 – 6.7 Stable up to 40°C Low
HEPES 6.8 – 8.2 Stable up to 37°C Moderate
PIPES 6.1 – 7.5 Stable up to 30°C High

Detailed Applications of MES Buffer

Biochemical Research

MES is widely used in electrophoresis (SDS-PAGE) for protein separation due to its low UV absorption and minimal interference with enzymatic reactions.

Pharmaceutical Formulations

As a non-toxic buffering agent in ophthalmic solutions and parenteral drugs, maintaining pH stability during sterilization processes.

Molecular Biology

Critical for RNA and DNA hybridization techniques where precise pH control between 5.5-6.5 prevents nucleic acid degradation.

Industrial Applications

Used in biocatalysis processes requiring metal-ion-free environments for enzyme stability in bioreactors.

Usage Guidelines

Preparation Protocol

  1. Dissolve 21.9g MES in 800ml deionized water
  2. Adjust pH with NaOH/HCl to desired value (5.5-6.7)
  3. Bring final volume to 1L with DI water
  4. Filter sterilize using 0.22μm membrane

Storage Recommendations

  • Store unopened containers at 2-8°C
  • Use prepared solutions within 30 days
  • Avoid repeated freeze-thaw cycles

Safety Precautions

  • Wear PPE (gloves, goggles) when handling powder
  • In case of eye contact: Rinse immediately with water for 15 minutes
  • Emergency contact: (86) 15866781826

Real-World Case Studies

Case Study 1: Biotech Startup – ABC Laboratories

Challenge Protein aggregation during purification
Solution Implemented MES buffer (50mM, pH 6.0) in affinity chromatography
Result 95% monomeric protein yield, 30% faster process time

Case Study 2: Pharmaceutical Manufacturer – XYZ Pharma

Issue Drug precipitation in IV formulations
Approach Used MES buffer system (100mM, pH 6.2) with thermal stability testing
Outcome Stable formulation through autoclaving (121°C, 15psi)

Request Customized Solutions

Our technical team can provide:

  • Bulk quantities (25kg drums available)
  • GMP-grade certification
  • Custom buffer formulations
Contact our specialists:
Email: info@vivalr.com
Phone: (86) 15866781826
Available 24/7 for technical support
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