Table of Contents
- Product Information & Specifications
- Detailed Applications
- Usage Guidelines
- Case Studies
- Contact for Quotation
4-Morpholineethanesulfonic Acid (CAS 4432-31-9): Product Information
Basic Specifications
| Parameter | Details |
|---|---|
| CAS Number | 4432-31-9 |
| Molecular Formula | C6H13NO4S |
| Molecular Weight | 219.24 g/mol |
| Appearance | White crystalline powder |
| Solubility | Water-soluble (1M solution at 25°C) |
| pH Range | 5.5 – 6.7 |
| Storage | 2-8°C in dry conditions |
Comparative Analysis with Similar Buffers
| Buffer | pH Range | Temperature Stability | Metal Chelation |
|---|---|---|---|
| MES | 5.5 – 6.7 | Stable up to 40°C | Low |
| HEPES | 6.8 – 8.2 | Stable up to 37°C | Moderate |
| PIPES | 6.1 – 7.5 | Stable up to 30°C | High |
Detailed Applications of MES Buffer
Biochemical Research
MES is widely used in electrophoresis (SDS-PAGE) for protein separation due to its low UV absorption and minimal interference with enzymatic reactions.
Pharmaceutical Formulations
As a non-toxic buffering agent in ophthalmic solutions and parenteral drugs, maintaining pH stability during sterilization processes.
Molecular Biology
Critical for RNA and DNA hybridization techniques where precise pH control between 5.5-6.5 prevents nucleic acid degradation.
Industrial Applications
Used in biocatalysis processes requiring metal-ion-free environments for enzyme stability in bioreactors.
Usage Guidelines
Preparation Protocol
- Dissolve 21.9g MES in 800ml deionized water
- Adjust pH with NaOH/HCl to desired value (5.5-6.7)
- Bring final volume to 1L with DI water
- Filter sterilize using 0.22μm membrane
Storage Recommendations
- Store unopened containers at 2-8°C
- Use prepared solutions within 30 days
- Avoid repeated freeze-thaw cycles
Safety Precautions
- Wear PPE (gloves, goggles) when handling powder
- In case of eye contact: Rinse immediately with water for 15 minutes
- Emergency contact: (86) 15866781826
Real-World Case Studies
Case Study 1: Biotech Startup – ABC Laboratories
| Challenge | Protein aggregation during purification |
|---|---|
| Solution | Implemented MES buffer (50mM, pH 6.0) in affinity chromatography |
| Result | 95% monomeric protein yield, 30% faster process time |
Case Study 2: Pharmaceutical Manufacturer – XYZ Pharma
| Issue | Drug precipitation in IV formulations |
|---|---|
| Approach | Used MES buffer system (100mM, pH 6.2) with thermal stability testing |
| Outcome | Stable formulation through autoclaving (121°C, 15psi) |
Request Customized Solutions
Our technical team can provide:
- Bulk quantities (25kg drums available)
- GMP-grade certification
- Custom buffer formulations
Email: info@vivalr.com
Phone: (86) 15866781826
Available 24/7 for technical support



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