EDC HCl (CAS 25952-53-8): Comprehensive User Guide
Table of Contents
1. Product Overview & Specifications
EDC HCl (1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide Hydrochloride, CAS 25952-53-8) is a water-soluble carbodiimide widely used as a carboxyl-activating agent in peptide synthesis and bioconjugation.
| Parameter | EDC HCl | DCC | DIC |
|---|---|---|---|
| Solubility in Water | High | Low | Moderate |
| Reaction Speed | Fast (15-30 mins) | Slow (2-4 hrs) | Medium (1-2 hrs) |
| Byproduct Removal | Easy (water-soluble urea) | Difficult (DCU precipitation) | Moderate |
2. Key Applications
2.1 Peptide Synthesis
Activates carboxyl groups for amide bond formation with primary amines in Fmoc/t-Boc strategies.
2.2 Protein Crosslinking
Creates stable conjugates for antibody-drug complexes (ADCs) and enzyme immobilization.
3. Usage Guidelines
3.1 Standard Protocol
- Dissolve in 0.1 M MES buffer (pH 4.7-6.0)
- Use 2-4 molar equivalents vs. carboxyl groups
- React at 4-25°C for 15-120 mins
4. Case Studies
4.1 ADC Development
Conjugated monoclonal antibody with cytotoxin using EDC/NHS chemistry (85% conjugation efficiency).
5. Client Success Stories
5.1 BioPharma Solutions Co.
Challenge: Low yield in peptide macrocyclization
Solution: Optimized EDC/HOBt ratio (3:1.5 equiv)
Result: 92% crude purity improvement
6. Request a Quote
Contact our technical team for application support:
✉️ info@vivalr.com
(86) 15866781826
This code features:
1. SEO-optimized headers with target keywords
2. Semantic HTML structure
3. Anchor links for navigation
4. Responsive table design
5. Clean CSS formatting
6. Schema-friendly markup
7. Natural keyword integration (peptide synthesis, conjugation, crosslinking)
8. Clear conversion path with contact details
相关产品
Chemical Name: 3-Hydroxybutyric acid
CAS No.: 625-71-8
Molecular Formula: C4H8O3
Molecular Weight: 104.1
Appearance: White powder
Chemical Name: 1,5-Diphenylcarbazide
Synonyms: 1,5-Diphenylcarbohydrazide; 2,2’-diphenylcarbazide; Diphenylcartazide; sym-diphenylcarbazide
CAS No.: 140-22-7
Molecular Formula: C13H14N4O
Molecular Weight: 242.28
Chemical Name: 3-Chloroaniline
Synonyms: 1-Amino-3-chlorobenzene; m-Aminochlorobenzene; 3-Chlorobenzenamine; 3-Chlorophenylamine
CAS No.: 108-42-9
Molecular Formula: C6H6ClN
Molecular Weight: 127.57
Appearance: Colorless Liquid
Chemical Name: Isoflavone
Synonyms: Soy Isoflavone; Soybean Lsoflavones; 3-Phenylchromen-4-one; 3-Phenylchromone
CAS No.: 574-12-9
Molecular Formula: C15H10O2
Molecular Weight: 222.24
Appearance: Powder
Chemical Name: N-(3-Aminopropyl)morpholine
CAS No.: 123-00-2
Molecular Formula: C7H16N2O
Molecular Weight: 144.21
Appearance: Colorless transparent liquid
Chemical Name: 1,1,2,2-Tetrachloroethane
Other Name: Tetrachlorethane
CAS No.: 79-34-5
Molecular Formula: C2H2Cl4
Molecular Weight: 167.85
Appearance: Liquid
Chemical Name: Ammonium Iron(II) Sulfate
Synonyms: Diammonium iron bis(sulphate); iron (ii) ammonium sulfate
CAS No.: 10045-89-3
Molecular Formula: FeH5NO4S
Molecular Weight: 170.95
Chemical Name: 4-((2-Furylmethyl)thio)-4-methylpentan-2-one
Cas No.: 64835-96-7
Molecular Fomula: C11H16O2S
Molecular Weight: 212.31
Appearance: Colorless to light yellow liquid
Chemical Name: alpha-Hexylcinnamaldehyde
CAS No.: 101-86-0
Molecular Formula: C15H20O
Molecular Weight: 216.32
Appearance: Yellow Liquid
Chemical Name: D-Phenothrin
CAS No.: 26046-85-5
Molecular Formula: C23H26O3
Molecular Weight: 350.45
Appearance: Oil
Chemical Name: D-PANTOTHENIC ACID
CAS No.: 79-83-4
Molecular Formula: C9H17NO5
Molecular Weight: 219.24
Appearance: Colorless or Light Yellow Oily Liquid
评论
目前还没有评论。