MOPS Sodium Salt (Cas 71119-22-7): Comprehensive User Guide
Table of Contents
1. Product Overview & Specifications
MOPS Sodium Salt (Cas 71119-22-7)
A zwitterionic biological buffer widely used in biochemical and cell culture applications. Effective pH range: 6.5-7.9.
| Parameter | MOPS Sodium Salt | Alternative Buffers (e.g., Tris) |
|---|---|---|
| CAS Number | 71119-22-7 | 77-86-1 |
| Effective pH Range | 6.5 – 7.9 | 7.0 – 9.0 |
| Solubility (25°C) | >500 g/L in H2O | ~550 g/L |
| Cell Membrane Permeability | Low | High |
2. Key Applications
Core Uses in Research & Industry
- Cell Culture: Maintains stable pH in CHO and HEK293 cell lines
- Protein Purification: Buffer for ion-exchange chromatography
- Molecular Biology: Component of electrophoresis and PCR buffers
- Diagnostics: Stabilizer in enzymatic reaction kits
3. Usage Guidelines
Recommended Protocols
Typical Concentration: 10-50 mM in aqueous solutions
Preparation Example:
For 1L of 1M MOPS Sodium Salt solution:
1. Dissolve 231.2 g in 800 mL DI water
2. Adjust pH to 7.0 with NaOH/HCl
3. Bring volume to 1L
4. Case Studies
Real-World Implementations
| Application | Result |
|---|---|
| CHO Cell Culture Medium | 20% increase in monoclonal antibody yield |
| qPCR Buffer Optimization | Reduced non-specific binding by 35% |
5. Client Success Stories
ABC Biotech (Shanghai)
Challenge: Protein degradation during purification
Solution: 50 mM MOPS buffer in affinity chromatography
Outcome: 99% target protein recovery rate
XYZ Pharmaceuticals (Boston)
Challenge: pH fluctuations in bioreactors
Solution: MOPS-based buffered media
Outcome: Consistent 7.2±0.1 pH for 72hr culture
6. Contact for Technical Queries
Email: info@vivalr.com
Phone: (86) 15866781826
Request custom formulation or bulk pricing:
- OEM services available
- GMP-grade batches upon request
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