Pyranine (CAS 6358-69-6) User Guide: Applications, Case Studies & Technical Specifications
Table of Contents
1. Product Overview & Specifications
| Parameter | Pyranine (CAS 6358-69-6) | Conventional Fluorescent Dyes |
|---|---|---|
| Chemical Name | 8-Hydroxypyrene-1,3,6-trisulfonic acid trisodium salt | Varies by type |
| Molecular Weight | 524.36 g/mol | 300-600 g/mol |
| Excitation/Emission | 403/512 nm | 350-600 nm range |
| Quantum Yield | ≥0.85 (in aqueous solution) | 0.3-0.7 typical |
| pH Stability | 4.0-10.0 | Limited ranges |
2. Key Applications of Pyranine
2.1 Biological Research Applications
- Intracellular pH monitoring
- Membrane permeability studies
- Ion transport visualization
2.2 Industrial Monitoring Solutions
- Water treatment process control
- Hydraulic system leakage detection
- Polymerization reaction tracking
3. Usage Guidelines & Best Practices
3.1 Preparation Protocol
- Prepare 1mM stock solution in deionized water
- Filter through 0.22 μm membrane
- Store at 4°C protected from light
3.2 Calibration Procedure
Use pH standard buffers (4.0-9.0) for sensor calibration. Maintain temperature at 25±0.5°C during measurements.
4. Industry Case Studies
Case Study 1: Municipal Water Authority
| Client | Shanghai Water Management Bureau |
| Challenge | Detecting pipeline leaks in aging infrastructure |
| Solution | 0.1% Pyranine tracer injection with UV monitoring |
| Result | 92% leak detection accuracy improvement |
Case Study 2: Biomedical Research
| Client | National Institute of Biological Sciences |
| Application | Real-time lysosomal pH monitoring |
| Concentration | 50 μM working solution |
| Outcome | Published in Cell Metabolism (2023) |
5. Technical Comparison with Alternatives
| Feature | Pyranine | Fluorescein | Rhodamine B |
|---|---|---|---|
| Photostability | ★★★★☆ | ★★☆☆☆ | ★★★☆☆ |
| Aqueous Solubility | ＞100 mg/mL | 30 mg/mL | 5 mg/mL |
| pH Sensitivity | 0.1 pH unit | 0.3 pH unit | N/A |
6. Request Customized Solutions
For technical specifications or bulk pricing:
Email: info@vivalr.com
Tel: (86) 15866781826
Request SDS documentation or application notes:
- Custom concentration formulations
- GMP-grade production options
- Technical support packages
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