1. Product Overview & Specifications

Chemical Profile

• CAS Number: 92-31-9
• Molecular Formula: C₁₅H₁₆N₃SCl
• Molecular Weight: 305.83 g/mol
• Appearance: Dark green crystalline powder with bronze luster
• Solubility: Water (38.2 mg/mL), ethanol (4 mg/mL), methanol
• Storage: Room temperature, protected from light

Comparative Analysis

2. Key Applications

Histological Staining

• Nissl Bodies: Visualizes neuronal RNA in neuropathology
• Mast Cells: Identifies granules through metachromatic shift to purple-red
• Cartilage: Highlights proteoglycans in osteoarthritis studies

Diagnostic Medicine

• Oral cancer screening via mucosal staining
• Corneal injury assessment in ophthalmology
• DNA quantification in histochemistry

3. Step-by-Step Protocols

Mast Cell Staining Protocol

1. Deparaffinize sections to distilled water
2. Immerse in 0.1% Toluidine Blue (pH 2.0-2.5) for 4 minutes
3. Differentiate with 0.1% acetic acid (10 sec)
4. Dehydrate through ethanol series (70%→100%)
5. Clear in xylene and mount

Cytological Smear Protocol

1. Fix air-dried smears in methanol (5 min)
2. Stain with 0.05% Toluidine Blue (2 min)
3. Rinse in PBS (pH 7.4)
4. Air-dry and analyze under oil immersion

4. Case Studies

Plant Pathology Research

Objective: Analyze vascular changes in TuMV-infected plants
Method: 1-2 μm sections stained with 0.1% solution
Result: Distinct xylem/phloem disorganization in infected specimens

Cancer Diagnostics

Application: Oral lesion screening in 120 patients
Outcome: 94% specificity in malignant cell detection
Reference: J. Histotechnol. 17:357-359 (1994)

5. Client Success Stories

BioFount Technologies

Use Case: Viral infection studies in Arabidopsis
Feedback: “Superior resolution of cell wall structures compared to traditional dyes”

Shanghai MedLab Diagnostics

Application: Routine histopathology screening
Volume: 500+ slides processed monthly
Result: 30% reduction in false-positive rates