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Chemical Name: Toluidine Blue O
Synonyms: Dimethyltoluthionine chloride; Basic Blue 17; Methylene Blue T50
MF: C15H16ClN3S
MW: 305.82
CAS: 92-31-9
Appearance: Dark green to black powder
Assay: ≥85.0%

Samples: Available / Stock: Sufficient
Estimated delivery time: 3-5 days

Toluidine Blue O (CAS 92-31-9): Comprehensive User Guide

Table of Contents

1. Product Overview & Specifications

Chemical Profile

  • CAS Number: 92-31-9
  • Molecular Formula: C15H16N3SCl
  • Molecular Weight: 305.83 g/mol
  • Appearance: Dark green crystalline powder with bronze luster
  • Solubility: Water (38.2 mg/mL), ethanol (4 mg/mL), methanol
  • Storage: Room temperature, protected from light

Comparative Analysis

Feature Toluidine Blue O Methylene Blue Azure B
Metachromatic Properties Strong (purple-red for acidic components) Weak Moderate
Nucleic Acid Staining High affinity Moderate High
Clinical Diagnostics Cancer screening approved Limited Research use only

2. Key Applications

Histological Staining

  • Nissl Bodies: Visualizes neuronal RNA in neuropathology
  • Mast Cells: Identifies granules through metachromatic shift to purple-red
  • Cartilage: Highlights proteoglycans in osteoarthritis studies

Diagnostic Medicine

  • Oral cancer screening via mucosal staining
  • Corneal injury assessment in ophthalmology
  • DNA quantification in histochemistry

3. Step-by-Step Protocols

Mast Cell Staining Protocol

  1. Deparaffinize sections to distilled water
  2. Immerse in 0.1% Toluidine Blue (pH 2.0-2.5) for 4 minutes
  3. Differentiate with 0.1% acetic acid (10 sec)
  4. Dehydrate through ethanol series (70%→100%)
  5. Clear in xylene and mount

Cytological Smear Protocol

  1. Fix air-dried smears in methanol (5 min)
  2. Stain with 0.05% Toluidine Blue (2 min)
  3. Rinse in PBS (pH 7.4)
  4. Air-dry and analyze under oil immersion

4. Case Studies

Plant Pathology Research

Objective: Analyze vascular changes in TuMV-infected plants
Method: 1-2 μm sections stained with 0.1% solution
Result: Distinct xylem/phloem disorganization in infected specimens

Cancer Diagnostics

Application: Oral lesion screening in 120 patients
Outcome: 94% specificity in malignant cell detection
Reference: J. Histotechnol. 17:357-359 (1994)

5. Client Success Stories

BioFount Technologies

Use Case: Viral infection studies in Arabidopsis
Feedback: "Superior resolution of cell wall structures compared to traditional dyes"

Shanghai MedLab Diagnostics

Application: Routine histopathology screening
Volume: 500+ slides processed monthly
Result: 30% reduction in false-positive rates

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This is William, CEO of fousi chemical Chemical Co., Ltd.

Welcome to visit our factory . As the top chemical manufacturer, we have been striving for product quality, innovation , R&D, and customer service for the past 7 years. In the next 10 years or even longer, we are committed to becoming the most reliable chemical supplier in the world , creating a well-known international brand trusted by customers, with the spirit of “one meter wide, ten thousand meters deep”, we continue to focus on product research and development, continue to focus on customer service, continue to improve the supply chain service system, to create a professional chemical supply service team, to achieve win-win long-term cooperation. Please feel free to contact us if you have any questions.

Drug impurities to intermediates. Years of expertise.

Experience in the synthesis of complex compounds derived from American technology
Focus on the synthesis of difficult compounds
ISO 17034 dual system certification
National high-tech enterprise
5000+ square meters R&D center
A 70+ R&D team led by Chinese and foreign doctors
150 pieces (sets) of R&D equipment, 120+ fume hoods
18 patents

Solid QMS. Offer top-quality products and services.

Through more than ten years of continuous technological innovation and experience accumulation, the team's R&D personnel have established a complete quality management system for CATO, and have successively obtained ISO9001, ISO17034, CNAS and other international certifications.
It has successfully passed on-site inspections from the United States, Australia, Poland, Japan and domestic pharmaceutical company customers and institutions many times, providing high-quality one-stop services for R&D and production of new drugs around the world.

More than 150 sets of equipment to ensure quality.

The emphasis on quality is the basis for our survival and development, and is the most basic requirement for the products and services we provide. CATO has established a scientific, strict and comprehensive quality prevention and assurance system in accordance with international quality standards. Through advanced testing equipment such as GC-MS, chromatograph, nuclear magnetic resonance, scanning electron microscope, and ultraviolet light, quality management is implemented into every aspect of production and operation activities such as raw materials, inventory, production, testing, sales, verification, and training, and "All actions are controlled and traceable" as the work code of conduct for CATO employees.

Over 1000 cases. Synthesize complex compounds.

Currently, CATO serves more than 500 pharmaceutical companies, biotechnology and other companies around the world. Project types cover the entire cycle from pre-clinical to commercialization stages. As of 2020, CATO has delivered more than 1,000 projects in total.
The company has core technologies in key core technology fields such as high-end fluorine chemistry, asymmetric chiral synthesis, glycoside and biological enzyme technology, and continuous microreactors.

About the company

International Standard Chemicals Sell To All Over The World

FAQ

Does it support auditing?

Support, please make an appointment one day in advance. Company address: Room 716, Building 3, Ligao International Garden, No.1222 West Aoti Road, Lixia District, Jinan City, Shandong Province, P.R. China

Whether to provide samples?

Free samples can be provided for low value products, and small packaging specifications can be purchased for high value products.

Delivery time

Under normal circumstances, all items are in stock and will be shipped before 4 o'clock on the same day. Some products are produced to order, depending on the workshop situation and production schedule.

What is the production capacity?

At present, the company's workshop production equipment is mainly based on laboratories and pilot lines, with a maximum daily production capacity of 1.5 tons.

Can additional test indicators be added?

We can provide moisture, UV, IR, HNMR, CNMR, two-dimensional nuclear magnetic spectroscopy, optical rotation detection, elemental analysis, TGA analysis and residue analysis and other related tests to support personalized needs.

Technical direction

In the early stage, our company mainly engaged in drug impurity synthesis and standard product production, with core technologies in chirality, fluorine chemistry, and heterocycles.

Why Chem-iso’s delivery rate is guaranteed to be 99.99%

5000+

square meter R&D center

150+

China(set) of R&D equipment

150+

Fume hood

180+

R&D team

User’evaluation of us

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Michael Cooper

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James Black

" Sincere Chemical team visited our USA factory in the year of 2017. We think highly of cooperation with them. We thanked you for the past excellent orders and best product quality. We also talked about some new products for future orders. We will be still in strategic cooperation in the future and help each other."

Lisa Goodwill

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