BES sodium CAS 66992-27-6

Chemical Name: BES sodium
CAS No.: 66992-27-6
Molecular Fomula: C6H14NNaO5S
Molecular weight: 235.23
Appearance: White crystalline powder
Assay: ≥99%

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Comprehensive Product Guide: BES Sodium (CAS 66992-27-6)

Table of Contents

1. Product Specifications & Comparative Analysis

Parameter BES Sodium HEPES
CAS Number 66992-27-6 7365-45-9
Molecular Formula C6H14NNaO5S C8H18N2O4S
Molecular Weight 235.23 g/mol 238.30 g/mol
Effective pH Range 6.4-7.8 6.8-8.2
pKa (20°C) 7.15 7.5
Solubility 350 mg/mL in H2O >500 mg/mL
Purity Grade >99% (Molecular Biology) >99.5%

2. Key Applications & Industrial Uses

Core Functional Applications:

  • Molecular Biology: Essential buffer for PCR optimization and enzyme reactions
  • Diagnostic Testing: Stabilizing agent in clinical immunoassays
  • Cell Culture: Maintains physiological pH in mammalian cell systems
  • Protein Research: Electrophoresis buffer component for protein analysis

3. Operational Guidelines & Best Practices

  • Storage: Maintain at 4°C in sealed containers with desiccant
  • Solution Preparation: Use ultrapure water (18.2 MΩ·cm resistivity)
  • Concentration Range: 10-50 mM for most biological applications
  • Temperature Sensitivity: ±0.02 pH units/°C variation

4. Case Studies: Successful Implementations

Case 1: Vaccine Development Platform

Challenge: pH instability in viral vector purification
Solution: Implemented 25 mM BES sodium buffer system
Outcome: 32% yield improvement with maintained bioactivity

Case 2: High-Throughput Screening

Challenge: Fluorescence interference in HTS assays
Solution: BES-based buffer replacement
Outcome: 89% reduction in background noise

5. Verified Client Implementations

Organization Application Volume Duration
Shanghai Bioesn Biotech Diagnostic Kit Production 150 kg/yr 2019-Present
MedChemExpress (USA) Drug Discovery Research 25 kg/quarter 2022-Present
Canspec Scientific Academic Research Support 500+ institutions 2016-Present

6. Technical Consultation & Quotation

For technical specifications, bulk pricing, or formulation assistance:
Email: info@vivalr.com
Tel: (86) 15866781826

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