PROTEASE ALKALINE CAS 9014-01-1

Chemical Name: PROTEASE ALKALINE
CAS No.: 9014-01-1
Appearance: Clear transparent brown liquid
Assay: 200u/mg or 150u/mg or 8u/mg or 10u/mg

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Alkaline Protease (CAS 9014-01-1) Technical Guide

1. Product Specifications & Comparative Analysis

Parameter Specification Competitor A Competitor B
CAS Number 9014-01-1 9014-01-1 9014-01-1
Appearance White to light brown powder Off-white powder Beige powder
Enzyme Activity ≥150,000 U/g (adjustable to 200,000–1,500,000 U/g) 100,000 U/g 120,000 U/g
Optimal pH Range 9.0–11.0 8.5–10.5 9.0–11.5
Storage Conditions 2–8°C (dry), ≤-20°C for long-term Room temperature 4°C

Note: Custom formulations available for specific temperature/pH stability requirements.

2. Key Applications & Industrial Uses

Core Functionality

As a serine endopeptidase, Alkaline Protease hydrolyzes peptide bonds in proteins under alkaline conditions, enabling:

  • Detergent Industry: Removal of protein-based stains (blood, egg, grass) in laundry and dishwashing formulations.
  • Food Processing: Meat tenderization, brewing clarification, and dairy protein modification.
  • Leather & Textiles: Dehairing, bating, and silk degumming with reduced chemical use.
  • Biotechnology: Peptide synthesis in non-aqueous media (e.g., DMF) for pharmaceutical intermediates.

3. Operational Guidelines & Case Studies

Optimized Workflow

  1. Dosage: 0.2–0.5% (w/w) in detergents; adjust based on substrate complexity.
  2. Activation: Use at 40–60°C with pH 9.5–10.5 for 30–120 minutes.
  3. Inactivation: Heat to ≥75°C or lower pH to ≤6.0 post-reaction.

Case Example: Food Waste Valorization

A European biorefinery achieved 85% protein recovery from slaughterhouse waste using 0.3% Alkaline Protease at 55°C/pH 10, reducing processing time by 40% versus traditional methods.

4. Client Success Stories

Client A: Detergent Manufacturer (China)

  • Challenge: Improve cold-water stain removal in eco-friendly detergents.
  • Solution: Custom 400,000 U/g protease variant with enhanced low-temperature activity.
  • Outcome: 30% higher stain removal efficiency at 20°C vs. previous enzyme blends.

Client B: Pharmaceutical Supplier (USA)

  • Challenge: Synthesize glycosylated peptides without organic solvent denaturation.
  • Solution: Optimized protease-catalyzed reaction in DMF/water hybrid system.
  • Outcome: 92% yield improvement with 99% enantiomeric purity.

5. Contact for Custom Solutions

Request formulation support, bulk pricing, or technical documentation:

Email: info@vivalr.com
Tel: (86) 15866781826

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