Experience in the synthesis of complex compounds derived from American technology
Focus on the synthesis of difficult compounds
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A 70+ R&D team led by Chinese and foreign doctors
150 pieces (sets) of R&D equipment, 120+ fume hoods
18 patents
Alkaline Protease (CAS 9014-01-1) Technical Guide
Table of Contents
1. Product Specifications & Comparative Analysis
| Parameter | Specification | Competitor A | Competitor B |
|---|---|---|---|
| CAS Number | 9014-01-1 | 9014-01-1 | 9014-01-1 |
| Appearance | White to light brown powder | Off-white powder | Beige powder |
| Enzyme Activity | ≥150,000 U/g (adjustable to 200,000–1,500,000 U/g) | 100,000 U/g | 120,000 U/g |
| Optimal pH Range | 9.0–11.0 | 8.5–10.5 | 9.0–11.5 |
| Storage Conditions | 2–8°C (dry), ≤-20°C for long-term | Room temperature | 4°C |
Note: Custom formulations available for specific temperature/pH stability requirements.
2. Key Applications & Industrial Uses
Core Functionality
As a serine endopeptidase, Alkaline Protease hydrolyzes peptide bonds in proteins under alkaline conditions, enabling:
- Detergent Industry: Removal of protein-based stains (blood, egg, grass) in laundry and dishwashing formulations.
- Food Processing: Meat tenderization, brewing clarification, and dairy protein modification.
- Leather & Textiles: Dehairing, bating, and silk degumming with reduced chemical use.
- Biotechnology: Peptide synthesis in non-aqueous media (e.g., DMF) for pharmaceutical intermediates.
3. Operational Guidelines & Case Studies
Optimized Workflow
- Dosage: 0.2–0.5% (w/w) in detergents; adjust based on substrate complexity.
- Activation: Use at 40–60°C with pH 9.5–10.5 for 30–120 minutes.
- Inactivation: Heat to ≥75°C or lower pH to ≤6.0 post-reaction.
Case Example: Food Waste Valorization
A European biorefinery achieved 85% protein recovery from slaughterhouse waste using 0.3% Alkaline Protease at 55°C/pH 10, reducing processing time by 40% versus traditional methods.
4. Client Success Stories
Client A: Detergent Manufacturer (China)
- Challenge: Improve cold-water stain removal in eco-friendly detergents.
- Solution: Custom 400,000 U/g protease variant with enhanced low-temperature activity.
- Outcome: 30% higher stain removal efficiency at 20°C vs. previous enzyme blends.
Client B: Pharmaceutical Supplier (USA)
- Challenge: Synthesize glycosylated peptides without organic solvent denaturation.
- Solution: Optimized protease-catalyzed reaction in DMF/water hybrid system.
- Outcome: 92% yield improvement with 99% enantiomeric purity.
5. Contact for Custom Solutions
Request formulation support, bulk pricing, or technical documentation:
Email: info@vivalr.com
Tel: (86) 15866781826
