Chemical Name: 2′-Deoxyguanosine-5′-triphosphate trisodium salt
CAS No.: 93919-41-6
Molecular Fomula: C10H17N5NaO13P3
Molecular weight: 531.18
Appearance: white solid
This nucleotide analog is essential for molecular biology applications requiring DNA polymerase-mediated synthesis. As triphosphate sodium salt formulation, it offers enhanced solubility and stability for enzymatic reactions.
Parameter | Our Product | Vendor A | Vendor B |
---|---|---|---|
Molecular Formula | C10H16N5O13P3·3Na | C10H16N5O13P3·3Na | C10H16N5O13P3·3Na |
Purity (HPLC) | ≥99% | ≥98% | ≥97.5% |
Endotoxin Level | <0.05 EU/μmol | <0.1 EU/μmol | Not specified |
PCR Application: Use at final concentration of 200μM in reaction mix. Store working solutions at -20°C in aliquots. Avoid repeated freeze-thaw cycles (>3x).
Sequencing Reaction: Combine with dNTP mix in 1:3 molar ratio for optimal signal intensity. Verify pH stability between 7.0-7.5.
Challenge: Inconsistent sequencing results in high-GC regions
Solution: Implemented our dGTP at 1.5x concentration in ddNTP mix
Outcome: 92% improvement in base calling accuracy
Challenge: Low efficiency in CRISPR homology-directed repair
Solution: Optimized dNTP cocktail with our dGTP formulation
Outcome: HDR efficiency increased from 18% to 63%
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