Xylene Cyanole FF (CAS 2650-17-1) Comprehensive User Guide
Table of Contents
- Product Overview & Specifications
- Detailed Applications
- Usage Cases & Industry Best Practices
- Client Case Studies
- Request Quote or Technical Support
Product Overview & Specifications
| Parameter | Xylene Cyanole FF | Similar Tracking Dye (Comparison) |
|---|---|---|
| CAS Number | 2650-17-1 | N/A (Unique Identifier) |
| Molecular Formula | C25H27N2NaO6S2 | Varies by product type |
| Appearance | Dark green powder | Liquid/Powder variants |
| Electrophoresis Migration Rate | Approx. 50% bromophenol blue mobility | 30-70% range in alternatives |
| Storage Conditions | 2-8°C in dark containers | Room temp for stabilized solutions |
Detailed Applications
Core Industrial Uses
Xylene Cyanole FF serves as critical component in:
- DNA/RNA electrophoresis tracking systems
- Quality control dye for agarose gel preparation
- pH indicator in biochemical assays (effective range 0.2-2.8)
Technical Advantages
- Superior batch-to-batch consistency (≥98% purity)
- Low interference with downstream UV visualization
- Compatible with most common electrophoresis buffers
Usage Cases & Best Practices
Standard Protocol (0.4% Loading Buffer)
- Mix 4mg Xylene Cyanole FF with 1mL glycerol
- Combine with 1mL Tris-EDTA buffer (pH 8.0)
- Centrifuge at 10,000g for 5 minutes before use
Troubleshooting Guide
| Issue | Solution |
|---|---|
| Precipitation in cold storage | Warm to 25°C and vortex thoroughly |
| Band distortion | Verify dye concentration ≤0.04% |
Client Case Studies
Case 1: Genomics Lab Optimization
Client: Tier 1 European Biotech Firm
Challenge: Eliminate dye-induced RNA degradation
Solution: Implemented our ultra-pure Xylene Cyanole FF (99.2% purity)
Outcome: 40% reduction in sample loss, validated via Agilent Bioanalyzer
Case 2: High-Throughput Screening
Client: US Pharma Research Center
Challenge: Dye interference in automated systems
Solution: Custom low-fluorescence formulation
Outcome: Enabled 24/7 robotic operation with 99.8% detection accuracy
Request Product Information
Contact our technical team for:
- Bulk pricing (50kg+ orders)
- GMP-grade certification documents
- Custom formulation services
Email: info@vivalr.com
Phone: (86) 15866781826



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