Phenylmethylsulfonyl fluoride CAS 329-98-6

Phenylmethylsulfonyl Fluoride (PMSF) CAS 329-98-6 Product Guide

Table of Contents

• 1. Product Specifications & Comparative Analysis
• 2. Functional Applications
• 3. Usage Protocols
• 4. Industry Case Studies
• 5. Client Implementations
• 6. Technical Consultation

1. Product Specifications & Comparative Analysis

2. Functional Applications

2.1 Protease Inhibition Mechanism

PMSF irreversibly inhibits serine proteases through sulfonylation of active site residues. Effective against:

• Trypsin (IC₅₀ = 0.1 μM)
• Chymotrypsin (IC₅₀ = 0.05 μM)
• Thrombin (IC₅₀ = 1.2 μM)

2.2 Pharmaceutical Applications

• Protein extraction buffer additive
• Cell lysis solution component
• Diagnostic reagent stabilization

3. Usage Protocols

3.1 Laboratory Handling

Preparation of 100 mM stock solution:

1. Dissolve 174.2 mg PMSF in 10 ml isopropanol
2. Vortex until clear solution forms
3. Store at -20°C (6-month stability)

4. Industry Case Studies

4.1 Biologic Drug Production

Major European biopharma achieved 23% yield improvement in monoclonal antibody production through optimized PMSF concentration (0.8 mM) during cell harvest.

4.2 Diagnostic Kit Manufacturing

US-based IVD company reduced protease interference by 89% in ELISA kits using PMSF-containing preservation buffers.

5. Client Implementations

5.1 BioTech Solutions Inc. (2023)

• Application: Recombinant protein purification
• Challenge: 40% target protein degradation
• Solution: 0.5 mM PMSF in lysis buffer
• Outcome: 92% protein integrity maintained

5.2 PharmaCore Laboratories (2024)

• Application: Vaccine antigen preparation
• Challenge: Protease-induced epitope loss
• Solution: PMSF gradient optimization (0.3-0.7 mM)
• Outcome: Antigenicity preservation increased from 67% to 89%

6. Technical Consultation

Contact our protein chemistry experts for application-specific guidance:

Email: info@vivalr.com
Phone: (86) 15866781826

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• Concentration optimization calculator
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