Pyridoxal 5’-Phosphate (CAS 853645-22-4) Comprehensive Guide
Table of Contents
1. Product Specifications and Comparative Analysis
| Parameter | Pyridoxal 5’-Phosphate | Alternative Forms |
|---|---|---|
| CAS Number | 853645-22-4 | 54-47-7 (hydrate form) |
| Molecular Formula | C8H10NO6P | C8H12N2O2 (Pyridoxylamine) |
| Molecular Weight | 247.142 g/mol | 241.115 g/mol (HCl salt) |
| Purity | ≥98% (HPLC) | 99.76% (MCE standard) |
| Storage | -20°C (3 years stable) | 4°C (2 years stable) |
Key Features: Non-hazardous per GHS classification, water-soluble crystalline powder with 140-143°C melting point and 296°C flash point.
2. Key Applications and Functional Properties
Core Functions:
- Cofactor in 150+ enzymatic reactions including transamination and decarboxylation
- Essential component in amino acid metabolism and neurotransmitter synthesis
- Critical for hemoglobin formation and glycogen phosphorylase activation
Industrial Applications:
- Pharmaceuticals: Active ingredient in metabolic disorder treatments
- Biotechnology: Enzyme stabilization in diagnostic kits
- Research: Standard reference in HPLC and mass spectrometry analysis
3. Operational Guidelines and Best Practices
Handling Protocols:
- Reconstitute with deionized water at 1-5 mg/mL concentration
- Maintain pH 6.5-7.5 for optimal enzymatic activity
- Avoid prolonged exposure to light (>30 min)
Quality Control:
- HPLC purity verification with C18 column (retention time: 4.2±0.3 min)
- NMR validation (δ 8.1 ppm for aldehyde proton)
4. Industry-Specific Use Cases
Case A: Pharmaceutical Manufacturing
Implemented as a stabilizer in levodopa formulations, improving shelf-life by 38% under accelerated stability testing (40°C/75% RH).
Case B: Clinical Diagnostics
Used in alanine transaminase (ALT) test kits, demonstrating 99.2% correlation with Roche Diagnostics® reference methods.
5. Verified Customer Implementations
MedChemExpress (MCE)
Utilized in high-throughput screening of vitamin B6-dependent enzymes, achieving 92% assay reproducibility across 50,000+ compounds.
Shenzhen Hisan Biotechnology
Integrated into lateral flow immunoassays for homocysteine detection, reducing false-positive rates by 27% compared to conventional methods.
6. Technical Consultation and Procurement
For batch-specific COA, custom synthesis, or bulk pricing:
| Contact Method | Details |
|---|---|
| Technical Support | Email: info@vivalr.com Tel: (86) 15866781826 |
| Order Processing | Lead time: 2-3 weeks (standard) Express shipping available (DHL/FedEx) |



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