Hematoxylin CAS 517-28-2

Table of Contents

• Product Overview
• Specifications & Comparative Analysis
• Key Applications
• Usage Guidelines
• Industry Case Studies
• Technical FAQ
• Contact for Custom Solutions

Hematoxylin (CAS 517-28-2) Essential Profile

A natural phenolic compound extracted from logwood, Hematoxylin (C₁₆H₁₄O₆) serves as the critical nuclear stain in histology. This oxidation-dependent dye requires maturation with oxidizing agents for optimal staining performance.

Technical Specifications & Grade Comparison

Core Applications in Modern Science

Histological Staining

Forms the foundation of H&E staining protocols, enabling clear nuclear visualization in tissue sections with staining duration typically between 5-15 minutes depending on tissue type.

Cytological Analysis

Used in Papanicolaou stains for cervical cancer screening, requiring precise pH control (2.5-3.0) for optimal results.

Educational Kits

Pre-formulated solutions for classroom microscopy experiments with standardized 0.5% w/v concentrations.

Optimized Staining Protocol

1. Solution Preparation: Dissolve 1g hematoxylin in 10ml ethanol + 190ml DI water
2. Oxidation: Add 0.1g sodium iodate, stir for 48 hours
3. Acidification: Adjust to pH 2.3 with hydrochloric acid
4. Filter through 0.45μm membrane before use

Documented Implementation Cases

Case 1: University Hospital Pathology Lab

Challenge: Inconsistent nuclear staining across 5,000+ annual biopsy samples
Solution: Implemented pH-controlled hematoxylin batches (2.8±0.1)
Outcome: 92% reduction in staining variability

Case 2: Pharmaceutical QC Department

Challenge: Meeting FDA staining validation requirements
Solution: Custom-certified hematoxylin with lot-specific documentation
Result: Successful 21 CFR Part 11 compliance audit

Technical Support FAQ

Q: Why does hematoxylin solution turn precipitate?

A: Oxidation products form beyond 60-day shelf life – filter and replenish oxidizer monthly

Q: Optimal staining temperature range?

A: Maintain 22-25°C for consistent results (±2°C variation acceptable)

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Lead Time: 2-4 weeks for GMP-grade production