CHAPSO (CAS 82473-24-3) Technical Guide
Table of Contents
1. Product Overview & Specifications
| Parameter | Details |
|---|---|
| CAS Number | 82473-24-3 |
| Chemical Name | 3-[(3-Cholamidopropyl)dimethylammonio]-2-hydroxy-1-propanesulfonate |
| Molecular Formula | C32H58N2O8S |
| Molecular Weight | 630.88 g/mol |
| Appearance | White crystalline powder |
| Purity | ≥98% (Ultra Pure Grade) |
| Solubility | Water, ethanol, and ether |
Supplier Comparison
| Supplier | Specification | Price (500mg) |
|---|---|---|
| Yongjian Pharma | BioReagent Plus | ¥1,230 |
| Sigma-Aldrich (via Shanghai Yezhou Bio) | BioXtra Grade | ¥850 |
| Amresco (via Beijing Huamaike Bio) | Ultra Pure Grade | ¥590 |
2. Key Applications & Uses
- Membrane Protein Solubilization: Non-denaturing zwitterionic detergent for stabilizing integral membrane proteins.
- Cell Culture: Used in media formulations to maintain protein integrity.
- Electrophoresis & Chromatography: Enhances resolution in 2D gel electrophoresis and HPLC.
- Enzymology Studies: Preserves enzymatic activity during purification.
3. Usage Guidelines
Recommended Protocols
- Stock Solution: Prepare 10% (w/v) in ultrapure water, filter through 0.22µm membrane.
- Working Concentration: 0.1-2% (w/v) for protein extraction; optimize via titration.
- Storage: Stable at 2-8°C for 2 years; avoid freeze-thaw cycles.
4. Case Studies
Case 1: Structural Biology Research (2023)
Institution: Shanghai Institute of Biochemistry
Application: Solubilized G-protein coupled receptors (GPCRs) for cryo-EM analysis using 1.5% CHAPSO.
Result: Achieved 3.2Å resolution structure with retained ligand-binding activity.
Case 2: Pharmaceutical Development (2024)
Company: Jiangsu Yongjian Pharma
Application: Purified monoclonal antibodies using CHAPSO-based buffer (0.8% w/v).
Result: Increased yield by 22% compared to CHAPS detergent.
5. Supplier Comparison
| Parameter | Yongjian Pharma | Shanghai Yezhou Bio | Beijing Huamaike Bio |
|---|---|---|---|
| Lead Time | 3-5 days | 7 days | 5-7 days |
| Batch Certification | HPLC, MS | COA only | HPLC |
| Endotoxin Level | <0.005 EU/mg | Not specified | <0.01 EU/mg |
6. Contact for Quotations
Request custom quotes or technical support:
Email: info@vivalr.com
Tel: (86) 15866781826
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