Table of Contents
- Product Information & Specifications
- Detailed Applications
- Usage Guidelines
- Industry Case Studies
- Contact for Technical Consultation
Eosin Y (Acid Red 87) Technical Specifications
Basic Parameters
| Parameter | Specification | Eosin B Comparison | Fluorescein Comparison |
|---|---|---|---|
| CAS Number | 17372-87-1 | 548-26-5 | 2321-07-5 |
| Molecular Formula | C20H6Br4Na2O5 | C20H6Br2N2Na2O9 | C20H12O5 |
| Absorption Max | 515-518 nm | 514-517 nm | 494 nm |
| pH Stability | 4.0-6.0 | 3.5-5.5 | 6.5-8.0 |
Key Characteristics
- Water solubility: ≥50 mg/mL (20°C)
- Certified purity: ≥95% (HPLC)
- Storage conditions: 2-8°C in amber glass
- Certifications: ISO 9001, REACH, FDA 21 CFR 73.3110
Industrial & Scientific Applications
Histological Staining
- Nuclear counterstain in H&E staining protocols
- Cytoplasmic staining for epithelial cells
- Collagen visualization in connective tissues
Biomedical Research
- Apoptosis detection via fluorescence microscopy
- Cell viability assays (0.1-0.5% w/v solutions)
- Flow cytometry surface marker analysis
Industrial Uses
- Textile dyeing (silk/wool protein fibers)
- Ink formulations for security printing
- Laser dye component (520-550 nm range)
Optimal Usage Protocols
Histology Lab Procedure
- Prepare 1% alcoholic solution (70% ethanol)
- Stain sections for 2-3 minutes
- Differentiate in 70% ethanol + 1% HCl
- Dehydrate through ethanol series
Textile Dyeing Parameters
| Fiber Type | Temperature | Dye Concentration | Fixation Method |
|---|---|---|---|
| Silk | 60-70°C | 0.5-2% owf | Acetic acid bath |
| Wool | 80-85°C | 1-3% owf | Chromium mordant |
Documented Use Cases
Case Study 1: Diagnostic Lab Optimization
- Client: European Pathology Network (EPN)
- Challenge: Inconsistent H&E staining results
- Solution: Standardized Eosin Y formulation
- Outcome: 40% reduction in repeat staining
Case Study 2: Textile Manufacturing
- Client: Asian Silk Co., Ltd.
- Challenge: Fading in luxury fabric lines
- Solution: Mordant-modified Eosin Y
- Result: 5x improved washfastness
Case Study 3: Biotech Research
- Client: US Cancer Research Center
- Requirement: High-purity apoptosis marker
- Implementation: HPLC-validated batches
- Achievement: 99% assay reproducibility
Technical Consultation & Ordering
Product Options
| Package | Purity | Quantity | Lead Time |
|---|---|---|---|
| Lab Grade | ≥95% | 25g-1kg | 3-5 days |
| Research Grade | ≥99% | 100g-5kg | 7-10 days |
Contact Our Experts
Email: info@vivalr.com
Tel: (86) 15866781826
Request Custom Formulation
- Solvent-based preparations
- Buffered aqueous solutions
- GMP-grade manufacturing



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