1. Product Specifications & Comparative Analysis

2. Applications & Functional Roles

Clinical Diagnostics

• Liver Disease Monitoring: Quantifies liver damage in acute necrosis, cirrhosis, and obstructive jaundice.
• Enzyme-Linked Assays: Paired with urease for urea quantification in serum/plasma.

Biochemical Research

• Ammonia Detection: Catalyzes reversible conversion of L-glutamate to α-ketoglutarate.
• Metabolic Studies: Analyzes energy metabolism in hepatic and renal tissues.

3. Operational Protocols & Case Studies

Case Study 1: Liver Function Testing

Objective: Diagnose early-stage alcoholic hepatitis.

Method:

1. Prepare GLDH reagent (0.1 M Tris buffer, pH 8.5).
2. Incubate serum sample with NADH and α-ketoglutarate at 30°C.
3. Measure absorbance at 340 nm for 10 min.

Result: Elevated GLDH activity (≥25 IU/L) confirmed hepatocellular injury.

Case Study 2: Industrial Ammonia Quantification

Workflow:

1. Add GLDH to wastewater sample with NADPH.
2. Monitor NADPH oxidation at 30°C.
3. Calculate ammonia concentration via standard curve.

Outcome: Achieved 95% accuracy in ammonia detection (0.5–50 ppm).

4. Client Success Stories

Client A: Shanghai JinNing Biotech

Challenge: Required high-purity GLDH for liver toxicity assays.

Solution: Supplied lyophilized GLDH (CAS 9029-12-3) with ≥500 units/mg activity.

Result: Reduced assay variability by 30% and accelerated R&D timelines.

Client B: Nanjing DuoLai Biosciences

Challenge: Needed GLDH-urease coupled system for urea detection.

Solution: Customized enzyme blend with stabilized activity at 4°C.

Result: Achieved 99% specificity in clinical urea measurements.

5. Contact for Custom Solutions

For bulk orders, technical queries, or customized formulations:

• Email: info@vivalr.com
• Phone: (86) 15866781826