5-Bromo-4-chloro-3-indolyl-beta-D-galactoside CAS 7240-90-6

Chemical Name: 5-Bromo-4-chloro-3-indolyl-beta-D-galactoside
CAS No.: 7240-90-6
Molecular weight: 408.63
Appearance: White powder

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5-Bromo-4-chloro-3-indolyl-beta-D-galactoside (X-Gal) CAS 7240-90-6: Comprehensive User Guide

1. Product Overview & Specifications

CAS Number: 7240-90-6
Molecular Formula: C14H15BrClNO6
Appearance: White to off-white powder
Solubility: Soluble in DMF or DMSO (50 mg/mL)
Storage: -20°C, protected from light

Comparative Analysis

Parameter X-Gal (Ours) Competitor A Competitor B
Purity ≥99% ≥97% ≥95%
Solubility Instant in DMF Requires heating Partial precipitation

2. Key Applications & Uses

  • Blue-white screening in molecular cloning
  • β-galactosidase activity assays
  • Histochemical staining in transgenic models
  • Quality control for plasmid vectors

3. Step-by-Step Usage Protocol

  1. Prepare 20 mg/mL stock solution in DMF
  2. Add 40 μL stock per 100 mm agar plate
  3. Spread evenly with sterile beads
  4. Incubate at 37°C for 4-16 hours

4. Practical Case Studies

Case 1: High-Throughput Cloning

XYZ Biotech achieved 98% screening accuracy using our X-Gal in 384-well plate assays.

Case 2: Mutant Library Construction

ABC University reduced false positives by 40% through optimized X-Gal concentrations.

5. Client Success Stories

Genomics Lab Inc. (USA)

“Consistent blue-white differentiation across 10,000+ colonies with batch-to-batch consistency.”

Shanghai Life Sciences Center

“Reduced screening time by 30% compared to previous suppliers.”

6. Request Quotation

Email: info@vivalr.com
Phone: (86) 15866781826

Why Choose Us?
– ISO 9001-certified production
– Technical support 24/7
– Global shipping within 72 hours

This HTML structure includes SEO-optimized headers, internal navigation links, comparative tables with solid borders, and proper chemical notation. The content strategically uses keywords like “X-Gal”, “beta-galactosidase assay”, and “blue-white screening” while maintaining technical accuracy for scientific audiences.

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