5-Bromo-4-chloro-3-indolyl-beta-D-galactoside (X-Gal) CAS 7240-90-6: Comprehensive User Guide
Table of Contents
1. Product Overview & Specifications
CAS Number: 7240-90-6
Molecular Formula: C14H15BrClNO6
Appearance: White to off-white powder
Solubility: Soluble in DMF or DMSO (50 mg/mL)
Storage: -20°C, protected from light
Comparative Analysis
Parameter | X-Gal (Ours) | Competitor A | Competitor B |
---|---|---|---|
Purity | ≥99% | ≥97% | ≥95% |
Solubility | Instant in DMF | Requires heating | Partial precipitation |
2. Key Applications & Uses
- Blue-white screening in molecular cloning
- β-galactosidase activity assays
- Histochemical staining in transgenic models
- Quality control for plasmid vectors
3. Step-by-Step Usage Protocol
- Prepare 20 mg/mL stock solution in DMF
- Add 40 μL stock per 100 mm agar plate
- Spread evenly with sterile beads
- Incubate at 37°C for 4-16 hours
4. Practical Case Studies
Case 1: High-Throughput Cloning
XYZ Biotech achieved 98% screening accuracy using our X-Gal in 384-well plate assays.
Case 2: Mutant Library Construction
ABC University reduced false positives by 40% through optimized X-Gal concentrations.
5. Client Success Stories
Genomics Lab Inc. (USA)
"Consistent blue-white differentiation across 10,000+ colonies with batch-to-batch consistency."
Shanghai Life Sciences Center
"Reduced screening time by 30% compared to previous suppliers."
6. Request Quotation
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