Table of Contents
- Product Information & Specifications
- Detailed Applications
- Usage Guidelines
- Case Studies
- Contact for Custom Solutions
Deoxythymidine Triphosphate (dTTP) CAS 18423-43-3: Product Information & Specifications
Basic Parameters
| Parameter | Specification |
|---|---|
| CAS Number | 18423-43-3 |
| Molecular Formula | C10H17N2O14P3 |
| Molecular Weight | 502.15 g/mol |
| Purity | ≥99% (HPLC) |
| Storage | -20°C, desiccated |
Comparative Analysis of dTTP Grades
| Feature | Analytical Grade | Molecular Biology Grade |
|---|---|---|
| Endotoxin Level | <10 EU/mg | <1 EU/mg |
| DNase/RNase Activity | Not tested | Undetectable |
| Typical Applications | Basic research | PCR, sequencing |
Detailed Applications of dTTP in Biotechnology
Core Applications
- PCR Amplification: Critical component in DNA polymerase-driven amplification.
- DNA Sequencing: Used in Sanger sequencing for chain termination.
- cDNA Synthesis: Essential for reverse transcription processes.
- Site-Directed Mutagenesis: Facilitates precise genetic modifications.
Emerging Uses
- CRISPR-Cas9 genome editing workflows
- NGS library preparation
- Single-cell sequencing protocols
Optimal Usage Guidelines
Best Practices
- Maintain strict cold chain during transport
- Use nuclease-free buffers for dilution
- Avoid repeated freeze-thaw cycles
Common Pitfalls
- Contamination from non-sterile handling
- Degradation due to improper pH conditions
- Incorrect concentration in master mixes
Industry Case Studies
Case 1: Diagnostic Kit Development
Client: European Molecular Diagnostics Lab
Challenge: Required ultra-pure dTTP for COVID variant detection assays
Solution: Supplied molecular biology-grade dTTP with batch-specific QC reports
Outcome: 40% improvement in assay sensitivity
Case 2: Agricultural Genomics Research
Client: US Agri-Biotech Firm
Challenge: Needed stable dNTPs for field-deployable PCR systems
Solution: Custom lyophilized formulations with 18-month stability
Outcome: Enabled onsite crop pathogen detection
Request Customized Solutions
Contact our technical team for specialized formulations:
- Email: info@vivalr.com
- Phone: (86) 15866781826
Specify your requirements:
- Desired purity level
- Quantity requirements
- Application-specific needs



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