Deoxythymidine triphosphate CAS 18423-43-3

Chemical Name: Deoxythymidine triphosphate
Synonyms: 2′-Deoxythymidine 5′-triphosphate
CAS No.: 18423-43-3
Molecular Fomula: C10H18N2NaO14P3
Molecular weight: 506.17

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Table of Contents

Deoxythymidine Triphosphate (dTTP) CAS 18423-43-3: Product Information & Specifications

Basic Parameters

Parameter Specification
CAS Number 18423-43-3
Molecular Formula C10H17N2O14P3
Molecular Weight 502.15 g/mol
Purity ≥99% (HPLC)
Storage -20°C, desiccated

Comparative Analysis of dTTP Grades

Feature Analytical Grade Molecular Biology Grade
Endotoxin Level <10 EU/mg <1 EU/mg
DNase/RNase Activity Not tested Undetectable
Typical Applications Basic research PCR, sequencing

Detailed Applications of dTTP in Biotechnology

Core Applications

  • PCR Amplification: Critical component in DNA polymerase-driven amplification.
  • DNA Sequencing: Used in Sanger sequencing for chain termination.
  • cDNA Synthesis: Essential for reverse transcription processes.
  • Site-Directed Mutagenesis: Facilitates precise genetic modifications.

Emerging Uses

  • CRISPR-Cas9 genome editing workflows
  • NGS library preparation
  • Single-cell sequencing protocols

Optimal Usage Guidelines

Best Practices

  • Maintain strict cold chain during transport
  • Use nuclease-free buffers for dilution
  • Avoid repeated freeze-thaw cycles

Common Pitfalls

  • Contamination from non-sterile handling
  • Degradation due to improper pH conditions
  • Incorrect concentration in master mixes

Industry Case Studies

Case 1: Diagnostic Kit Development

Client: European Molecular Diagnostics Lab
Challenge: Required ultra-pure dTTP for COVID variant detection assays
Solution: Supplied molecular biology-grade dTTP with batch-specific QC reports
Outcome: 40% improvement in assay sensitivity

Case 2: Agricultural Genomics Research

Client: US Agri-Biotech Firm
Challenge: Needed stable dNTPs for field-deployable PCR systems
Solution: Custom lyophilized formulations with 18-month stability
Outcome: Enabled onsite crop pathogen detection

Request Customized Solutions

Contact our technical team for specialized formulations:

  • Email: info@vivalr.com
  • Phone: (86) 15866781826

Specify your requirements:

  • Desired purity level
  • Quantity requirements
  • Application-specific needs
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