Table of Contents
- HEPES Sodium Salt Overview
- Technical Specifications & Comparative Analysis
- Research Applications
- Protocol Implementation Guide
- Client Success Stories
- Request Customized Solutions
HEPES Sodium Salt (CAS 75277-39-3) Overview
Chemical Profile
| Parameter | Specification |
|---|---|
| Chemical Name | 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt |
| CAS Number | 75277-39-3 |
| Molecular Formula | C8H17N2NaO4S |
| Molecular Weight | 260.28 g/mol |
| Appearance | White crystalline powder |
| Purity | ≥99% (HPLC) |
| pH Range | 6.8-8.2 (1M solution) |
| Storage | 2-8°C in dry conditions |
Buffer System Comparison
| Characteristic | HEPES Sodium | Tris Buffer | PBS |
|---|---|---|---|
| Temperature Sensitivity | Low | High | Moderate |
| Metal Chelation | None | Moderate | None |
| Cell Culture Compatibility | Excellent | Poor | Good |
| Enzyme Activity Preservation | Superior | Variable | Average |
Core Research Applications
Biomedical Implementations
- Mammalian Cell Culture: Maintains pH stability in CO2-independent environments
- Protein Crystallography: Preserves tertiary structure during purification
- Molecular Biology: Optimal for PCR buffer formulations (25mM concentration)
- Electrophysiology: Standard component in patch-clamp solutions
Experimental Implementation Protocol
Standard Preparation Method
- Prepare 1M stock solution in ultrapure water
- Adjust to desired pH using NaOH/HCl
- Sterilize through 0.22μm filtration
- Store at 4°C for ≤6 months
Concentration Guidelines
| Application | Recommended Concentration |
|---|---|
| Cell Culture Media | 10-25mM |
| Protein Studies | 50-100mM |
| Electrophysiology | 5-10mM |
Client Research Outcomes
Case Study 1: XYZ Biotech
- Challenge: pH fluctuation in hybridoma culture
- Solution: 20mM HEPES supplementation
- Result: 34% increase in monoclonal antibody yield
Case Study 2: ABC Pharmaceuticals
- Challenge: Enzyme degradation during purification
- Solution: 50mM HEPES in chromatography buffer
- Result: 92% activity retention post-purification
Request Customized Technical Support
Contact our biochemistry specialists for application-specific formulations:
- Email: info@vivalr.com
- Phone: (86) 15866781826
Request bulk pricing or GMP-grade certification documentation.
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