Pyridoxal 5’-Phosphate Cas 853645-22-4

Chemical Name: Pyridoxal 5’-Phosphate
CAS No.:853645-22-4
Molecular Fomula: C8H10NO6P
Molecular weight: 247.14

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Pyridoxal 5’-Phosphate (CAS 853645-22-4) Comprehensive Guide

1. Product Specifications and Comparative Analysis

Parameter Pyridoxal 5’-Phosphate Alternative Forms
CAS Number 853645-22-4 54-47-7 (hydrate form)
Molecular Formula C8H10NO6P C8H12N2O2 (Pyridoxylamine)
Molecular Weight 247.142 g/mol 241.115 g/mol (HCl salt)
Purity ≥98% (HPLC) 99.76% (MCE standard)
Storage -20°C (3 years stable) 4°C (2 years stable)

Key Features: Non-hazardous per GHS classification, water-soluble crystalline powder with 140-143°C melting point and 296°C flash point.

2. Key Applications and Functional Properties

Core Functions:

  • Cofactor in 150+ enzymatic reactions including transamination and decarboxylation
  • Essential component in amino acid metabolism and neurotransmitter synthesis
  • Critical for hemoglobin formation and glycogen phosphorylase activation

Industrial Applications:

  • Pharmaceuticals: Active ingredient in metabolic disorder treatments
  • Biotechnology: Enzyme stabilization in diagnostic kits
  • Research: Standard reference in HPLC and mass spectrometry analysis

3. Operational Guidelines and Best Practices

Handling Protocols:

  • Reconstitute with deionized water at 1-5 mg/mL concentration
  • Maintain pH 6.5-7.5 for optimal enzymatic activity
  • Avoid prolonged exposure to light (>30 min)

Quality Control:

  • HPLC purity verification with C18 column (retention time: 4.2±0.3 min)
  • NMR validation (δ 8.1 ppm for aldehyde proton)

4. Industry-Specific Use Cases

Case A: Pharmaceutical Manufacturing

Implemented as a stabilizer in levodopa formulations, improving shelf-life by 38% under accelerated stability testing (40°C/75% RH).

Case B: Clinical Diagnostics

Used in alanine transaminase (ALT) test kits, demonstrating 99.2% correlation with Roche Diagnostics® reference methods.

5. Verified Customer Implementations

MedChemExpress (MCE)

Utilized in high-throughput screening of vitamin B6-dependent enzymes, achieving 92% assay reproducibility across 50,000+ compounds.

Shenzhen Hisan Biotechnology

Integrated into lateral flow immunoassays for homocysteine detection, reducing false-positive rates by 27% compared to conventional methods.

6. Technical Consultation and Procurement

For batch-specific COA, custom synthesis, or bulk pricing:

Contact Method Details
Technical Support Email: info@vivalr.com
Tel: (86) 15866781826
Order Processing Lead time: 2-3 weeks (standard)
Express shipping available (DHL/FedEx)

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