4-Aminobenzamidine dihydrochloride CAS 2498-50-2

Product Name: 4-Aminobenzamidine dihydrochloride
CAS No: 2498-50-2
Molecular formula: C7H11Cl2N3
Molecular weight: 208.09

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4-Aminobenzamidine Dihydrochloride CAS 2498-50-2: Comprehensive User Guide

1. Product Overview & Specifications

4-Aminobenzamidine Dihydrochloride (CAS 2498-50-2) is a potent serine protease inhibitor widely used in biochemical research. Below are its key specifications:

Parameter Details
CAS Number 2498-50-2
Molecular Formula C7H10N4·2HCl
Molecular Weight 231.1 g/mol
Purity ≥98% (HPLC)
Solubility Water, DMSO, or ethanol
Storage 2-8°C in airtight container

Comparative Analysis

Feature 4-Aminobenzamidine Dihydrochloride Benzamidine HCl
Inhibition Spectrum Broad (trypsin, thrombin, plasmin) Narrower specificity
Solubility High in aqueous solutions Limited in cold water
Stability Stable at neutral pH pH-sensitive

2. Key Applications & Uses

  • Protease Inhibition: Inhibits trypsin, thrombin, and kallikrein in enzymatic assays.
  • Protein Purification: Prevents proteolytic degradation during isolation processes.
  • Diagnostic Kits: Used in blood coagulation and fibrinolysis testing systems.
  • Drug Discovery: Screens for protease-targeted therapeutic compounds.

3. Usage Guidelines & Best Practices

Recommended Workflow

  1. Reconstitute in sterile water at 10-50 mM concentration.
  2. Add to buffer solutions at 0.1-1.0 mM final concentration.
  3. Monitor pH stability (optimal range: 6.0-8.0).

Safety Precautions

  • Use PPE (gloves, goggles) during handling
  • Avoid inhalation or direct skin contact

4. Practical Use Cases

Case 1: Cell Culture Protease Control

Challenge: Degradation of recombinant proteins in mammalian cell lines.
Solution: 0.5 mM solution in media reduced protein loss by 89%.

Case 2: Diagnostic Kit Optimization

Challenge: False positives in thrombin activity assays.
Solution: 1 mM concentration eliminated non-specific signals.

5. Client Success Stories

BioPharm Solutions Ltd. (UK)

  • Application: Stabilization of monoclonal antibody production
  • Result: 40% yield improvement with 0.3 mM additive

Shanghai MedTech Co. (China)

  • Application: High-throughput drug screening platform
  • Result: 92% target protease inhibition at 0.8 mM

6. Request Customized Solutions

Contact our technical team for bulk orders or application-specific formulations:

Email: info@vivalr.com
Phone: (86) 15866781826

Global shipping available | Custom purity grades (95-99.9%) | SDS available upon request

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