4-Aminobenzamidine Dihydrochloride CAS 2498-50-2: Comprehensive User Guide
Table of Contents
1. Product Overview & Specifications
4-Aminobenzamidine Dihydrochloride (CAS 2498-50-2) is a potent serine protease inhibitor widely used in biochemical research. Below are its key specifications:
| Parameter | Details |
|---|---|
| CAS Number | 2498-50-2 |
| Molecular Formula | C7H10N4·2HCl |
| Molecular Weight | 231.1 g/mol |
| Purity | ≥98% (HPLC) |
| Solubility | Water, DMSO, or ethanol |
| Storage | 2-8°C in airtight container |
Comparative Analysis
| Feature | 4-Aminobenzamidine Dihydrochloride | Benzamidine HCl |
|---|---|---|
| Inhibition Spectrum | Broad (trypsin, thrombin, plasmin) | Narrower specificity |
| Solubility | High in aqueous solutions | Limited in cold water |
| Stability | Stable at neutral pH | pH-sensitive |
2. Key Applications & Uses
- Protease Inhibition: Inhibits trypsin, thrombin, and kallikrein in enzymatic assays.
- Protein Purification: Prevents proteolytic degradation during isolation processes.
- Diagnostic Kits: Used in blood coagulation and fibrinolysis testing systems.
- Drug Discovery: Screens for protease-targeted therapeutic compounds.
3. Usage Guidelines & Best Practices
Recommended Workflow
- Reconstitute in sterile water at 10-50 mM concentration.
- Add to buffer solutions at 0.1-1.0 mM final concentration.
- Monitor pH stability (optimal range: 6.0-8.0).
Safety Precautions
- Use PPE (gloves, goggles) during handling
- Avoid inhalation or direct skin contact
4. Practical Use Cases
Case 1: Cell Culture Protease Control
Challenge: Degradation of recombinant proteins in mammalian cell lines.
Solution: 0.5 mM solution in media reduced protein loss by 89%.
Case 2: Diagnostic Kit Optimization
Challenge: False positives in thrombin activity assays.
Solution: 1 mM concentration eliminated non-specific signals.
5. Client Success Stories
BioPharm Solutions Ltd. (UK)
- Application: Stabilization of monoclonal antibody production
- Result: 40% yield improvement with 0.3 mM additive
Shanghai MedTech Co. (China)
- Application: High-throughput drug screening platform
- Result: 92% target protease inhibition at 0.8 mM
6. Request Customized Solutions
Contact our technical team for bulk orders or application-specific formulations:
Email: info@vivalr.com
Phone: (86) 15866781826
Global shipping available | Custom purity grades (95-99.9%) | SDS available upon request



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