Isopropyl-β-D-Thiogalactopyranoside (IPTG) CAS 367-93-1: Comprehensive User Guide

1. Technical Specifications & Parameter Comparison

2. Applications in Molecular Biology

IPTG serves as:

• Lac operon inducer in E. coli protein expression systems
• Critical component in blue-white screening for cloning
• Controlled gene expression in mammalian cell studies

3. Usage Protocols & Best Practices

Standard Induction Protocol:

1. Prepare 1M stock solution in sterile water
2. Use 0.1-1 mM final concentration in culture medium
3. Induce at OD₆₀₀ 0.4-0.6 for optimal results

4. Industry Use Cases

Pharmaceutical Production:

Used in GMP-compliant facilities for therapeutic antibody production with titers exceeding 5 g/L.

5. Customer Success Stories

Case 1: Biotech Startup (Shanghai, 2023)

Challenge: Low recombinant protein yield in BL21(DE3) strains
Solution: Optimized IPTG concentration gradient (0.2-0.8 mM)
Result: 300% yield improvement with 0.4 mM induction

Case 2: Vaccine Manufacturer (Germany, 2022)

Challenge: Inconsistent expression in large-scale fermentation
Solution: Pulse-fed IPTG delivery system
Result: Batch-to-batch consistency improved to 98%

6. Request Customized Solutions

Our technical team provides:

• Bulk quantities (1g to 100kg batches)
• GMP-grade certification support
• Custom formulation services