tris(hydroxymethyl)aminomethane hydrochloride cas 1185-53-1
Appearance: White crystalline powder
Purity: 100.28%
Package: 25kg/fiber drum, or as customized package
Sample: Available
related documents:
COA of Tris Hydrochloride 1185-53-1
TDS of Tris Hydrochloride 1185-53-1
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Table of Contents
- Product Overview & Key Specifications
- Detailed Applications in Life Sciences
- Industry Case Studies (2020-2024)
- Technical Selection Guide
- Request Custom Formulation
Tris HCl (CAS 1185-53-1) Product Overview
Chemical Specifications Comparison
| Parameter | Pharma Grade | Molecular Biology Grade | Industrial Grade |
|---|---|---|---|
| Purity | ≥99.5% | ≥99.0% | ≥98.0% |
| Endotoxin Level | <0.1 EU/mg | <0.5 EU/mg | N/A |
| pH Range (1M, 25°C) | 7.0-9.0 | 7.0-9.2 | 6.8-9.5 |
Key Technical Parameters
- Molecular Weight: 157.6 g/mol
- Buffer Capacity: 7.5-9.0 pH
- Solubility: 1M in H₂O (25°C)
- DNase/RNase Activity: Undetectable
Core Applications in Biotechnology
Protein Research Applications
- Western Blotting: 25mM Tris HCl buffer systems
- Chromatography: Affinity column equilibration
- Crystallization: pH stabilization in 8.0-8.5 range
Diagnostic Reagent Formulations
- PCR Master Mixes: 10-50mM concentration
- ELISA Wash Buffers: 0.05% Tween-20 modified
- Blood Chemistry Analyzers: pH control in enzymatic assays
Documented User Cases (2020-2024)
Case 1: mRNA Vaccine Production
Client: European CDMO (Confidential)
Application: Buffer system for in vitro transcription
Results: 22% increase in mRNA yield vs. phosphate buffers
Case 2: Biopharma Process Development
Client: Top 10 Pharma Company
Application: Downstream purification of mAbs
Results: 99.8% host cell protein removal
Case 3: Industrial Enzyme Manufacturing
Client: Asian Enzyme Producer
Application: Fermentation media stabilization
Results: 15% longer enzyme half-life
Technical Selection Matrix
| Application | Recommended Grade | Typical Concentration |
|---|---|---|
| Diagnostic Kits | Pharma Grade | 10-100mM |
| Research Use | Molecular Biology Grade | 5-200mM |
Request Customized Solutions
For bulk orders or specialized formulations:
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Phone: (86) 15866781826
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