3,3′-Diaminobenzidine Tetrahydrochloride Hydrate (CAS 868272-85-9) User Guide
Table of Contents
1. Product Specifications & Comparative Analysis
| Parameter | Specification |
|---|---|
| CAS Number | 868272-85-9 |
| Molecular Formula | C12H18Cl4N4·xH2O |
| Purity | ≥98% (HPLC) |
| Solubility | Water: 50 mg/mL |
| Storage | 2-8°C, protect from light |
Competitive Advantages vs Alternatives
| Feature | Our Product | Standard DAB |
|---|---|---|
| Stability | 24 months at 4°C | 12-18 months |
| Batch Consistency | ±2% variance | ±5-8% variance |
2. Core Applications in Biomedical Research
Primary Use Cases:
- Immunohistochemistry (IHC): HRP-based chromogenic detection
- Western Blotting: Membrane-based protein visualization
- Electron Microscopy: Heavy metal staining enhancement
Optimized Protocols
| Application | Recommended Concentration | Incubation Time |
|---|---|---|
| IHC Staining | 0.05% w/v | 3-7 minutes |
| Western Blot | 0.1% w/v | 5-10 minutes |
3. Implementation Case Studies
Case 1: Cancer Biomarker Research
Challenge: Low-abundance protein detection in FFPE tissues
Solution: Optimized DAB incubation protocol at 0.03% concentration
Result: 40% sensitivity improvement vs commercial alternatives
4. Verified Client Implementations
University of Cambridge (UK)
“Achieved consistent staining across 500+ brain tissue samples with ≤2% variance.”
Genentech Research Labs (USA)
“Reduced background noise by 62% in PD-L1 detection assays using optimized formulation.”
5. Technical Support & Ordering
Contact our biochemistry specialists:
Email: info@vivalr.com
Tel: (86) 15866781826
Why Choose Our Product?
- ISO 9001-certified production
- Custom concentration formulations
- 24/7 technical support
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