DL-1,4-dithiothreitol Cas 3483-12-3

Chemical Name:DL-1,4-Dithiothreitol
CAS No.: 3483-12-3
Molecular Formula: C4H10O2S2
Molecular weight: 154.25
Appearance: White free flowing powder,hygroscopic
Assay: 98%

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DL-1,4-Dithiothreitol (CAS 3483-12-3) Overview
Key Parameters & Comparative Analysis
Applications in Biochemical Research
Step-by-Step Usage Protocols
Industry Case Studies
Contact for Technical Consultation

DL-1,4-Dithiothreitol (CAS 3483-12-3) Overview

DL-1,4-Dithiothreitol (DTT), CAS 3483-12-3, is a potent reducing agent widely used to break disulfide bonds in proteins and stabilize enzymes. With molecular formula C₄H₁₀O₂S₂ and molar mass 154.25 g/mol, it operates effectively in pH ranges 6.0-9.0.

Key Parameters & Comparative Analysis

Parameter	DL-1,4-DTT	β-Mercaptoethanol	TCEP
Reduction Efficiency	High (2-electron donor)	Moderate	High (non-thiol)
Working pH	6.0-9.0	7.0-8.5	1.5-8.5
Odor	Mild	Strong	Odorless

Applications in Biochemical Research

1. Protein Denaturation: Breaks S-S bonds in SDS-PAGE sample prep.
2. Enzyme Stabilization: Maintains activity of sulfhydryl-dependent enzymes.
3. Nucleic Acid Studies: Prevents RNA/DNA degradation by nucleases.

Step-by-Step Usage Protocols

Western Blotting Protocol:
1. Prepare 1M DTT stock in 0.01M sodium acetate (pH 5.2)
2. Add 50-100mM final concentration to sample buffer
3. Heat samples at 95°C for 5 minutes before electrophoresis

Industry Case Studies

Case 1: PharmaTech Solutions (2023)
– Challenge: Protein aggregation in monoclonal antibody production
– Solution: 20mM DTT in purification buffer
– Outcome: 89% reduction in aggregate formation

Case 2: BioGenomics Ltd. (2024)
– Application: RNA sequencing library prep
– Protocol: 10mM DTT with RNase inhibitor
– Result: 98.7% RNA integrity maintained