immobilized lipase novozyme 435 cas 9001-62-1

Assay: 12000PLU
Capacity: 80kg/month
Packaging: 500g/Bag
Sample: Available
Appearance:White crystalline granular powder

related documents:

COA of Candida Antarctica Lipase
TDS of Candida Antarctica Lipase

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Comprehensive Guide to Immobilized Lipase Novozyme 435 (CAS 9001-62-1)

Table of Contents

1. Product Overview & Specifications

Immobilized Lipase Novozyme 435 (CAS 9001-62-1) is a commercially available Candida antarctica lipase B immobilized on a macroporous acrylic resin. It is widely used in esterification, transesterification, and hydrolysis reactions under mild conditions.

Key Specifications:

Parameter Value
CAS Number 9001-62-1
Source Candida antarctica Lipase B
Carrier Material Macroporous Acrylic Resin
Enzyme Activity ≥10,000 PLU/g
Optimal pH 6.0-8.0
Optimal Temperature 30-60°C
Storage Conditions 2-8°C (dry)

2. Parameter Comparison with Similar Products

Feature Novozyme 435 Lipozyme TL IM Lipase RM IM
Enzyme Origin C. antarctica Thermomyces lanuginosus Rhizomucor miehei
Carrier Stability High Medium Medium
Operational Stability ≥20 cycles 10-15 cycles 8-12 cycles
Substrate Specificity Broad Medium-chain TG Long-chain TG

3. Detailed Industrial Applications

3.1 Biodiesel Production

Catalyzes transesterification of triglycerides with methanol to produce fatty acid methyl esters (FAME).

3.2 Food Industry

  • Interesterification of oils to produce trans-free margarine
  • Synthesis of flavor esters (e.g., isoamyl acetate)

3.3 Pharmaceutical Synthesis

Enantioselective resolution of chiral alcohols/acids for drug intermediates.

4. Usage Guidelines & Best Practices

4.1 Storage & Handling

Store at 2-8°C in original container. Avoid moisture exposure.

4.2 Reaction Setup

  1. Optimize solvent system (recommended: tert-butanol or solvent-free)
  2. Use enzyme loading 5-20% (w/w of substrates)
  3. Maintain water activity <0.3 for esterification

5. Real-World Case Studies

5.1 Case Study: Biodiesel Manufacturer (EU)

Challenge: Achieve >96% FAME yield with waste cooking oil.
Solution: Novozyme 435 (12% loading) at 50°C for 8h.
Result: 98.2% conversion, 15 reuse cycles.

5.2 Case Study: Nutraceutical Company (USA)

Challenge: Produce Omega-3 enriched structured lipids.
Solution: Enzymatic acidolysis with Novozyme 435.
Result: 75% EPA/DHA incorporation, 90% purity.

6. Request Product Information

Contact our technical team for SDS, COA, or bulk pricing:

Request a free sample or application protocol today.

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