DIPSO (CAS 68399-80-4): Comprehensive Technical Guide
Table of Contents
1. Product Overview & Specifications
| Parameter | DIPSO (CAS 68399-80-4) |
|---|---|
| Chemical Name | 3-(Bis(2-hydroxyethyl)amino)-2-hydroxypropanesulfonic acid |
| Molecular Formula | C7H17NO6S |
| Molecular Weight | 243.28 g/mol |
| Appearance | White crystalline powder |
| Solubility | Water: 0.1M at 20°C |
| pH Range | 7.0 – 8.2 (effective buffering zone) |
| Melting Point | 189-192°C |
| LogP | -1.40 |
| PSA | 126.68 Ų |
2. Key Applications
DIPSO is a zwitterionic biological buffer with critical roles in:
- Cell culture media optimization
- Molecular biology protocols requiring pH stability
- Oocyte meiosis studies (mouse model applications)
- Protein purification workflows
- Enzyme activity assays in neutral-alkaline conditions
3. Usage Guidelines
Recommended Preparation
Dissolve 24.3g in 1L distilled water to prepare 0.1M solution. Filter sterilize (0.22μm) for cell culture use.
Critical Notes
- Maintain storage at 15-25°C in airtight container
- Avoid prolonged exposure to temperatures >40°C
- Not recommended for radical-containing systems
4. Performance Comparison
| Buffer | pH Range | Temperature Stability | Ionic Strength |
|---|---|---|---|
| DIPSO | 7.0-8.2 | ±0.02 pH/°C | Low |
| HEPES | 6.8-8.2 | ±0.03 pH/°C | Moderate |
| MOPS | 6.5-7.9 | ±0.01 pH/°C | Low |
5. Industry Case Studies
Case 1: Reproductive Biology Research
A Tier 1 university laboratory successfully utilized 10mM DIPSO solution to maintain pH stability during 72-hour oocyte maturation studies, achieving 92% viability rates.
Case 2: Pharmaceutical QC Testing
GMP facility implemented DIPSO-based buffer systems for monoclonal antibody characterization, reducing buffer-related interference in HPLC analyses by 38% compared to traditional buffers.
6. Contact for Technical Support
For formulation assistance or bulk order inquiries:
Email: info@vivalr.com
Tel: (86) 15866781826
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