5-Azacytosine: Comprehensive User Guide for Research and Procurement
Table of Contents
1. Product Overview & Specifications
| Parameter | 5-Azacytosine | 5-Aza-2′-Deoxycytidine | Zebularine |
|---|---|---|---|
| CAS Number | 320-67-2 | 2353-33-5 | 3690-10-6 |
| Molecular Formula | C4H5N3O5 | C8H12N4O5 | C9H12N2O5 |
| Mechanism | DNA/RNA methyltransferase inhibitor | DNA methylation inhibitor | Cytidine deaminase inhibitor |
2. Key Applications
Epigenetic Research
Demethylation studies for cancer biomarker discovery and gene silencing reversal.
Cancer Therapeutics
Used in hematologic malignancies (MDS, AML) at concentrations 0.5-10μM for 72hr exposure.
Plant Biology
Induces somaclonal variation in crops at 25-100μM treatment for 2-4 weeks.
3. Usage Guidelines
| Application | Concentration | Duration |
|---|---|---|
| Cell Culture Treatment | 1-10 μM | 48-96 hours |
| Plant Tissue Culture | 25-100 μM | 14-28 days |
4. Client Success Stories
Case 1: Cancer Research Center
Challenge: Reactivate tumor suppressor genes in lung adenocarcinoma
Solution: 5μM 5-Azacytosine + 0.5μM TSA for 72hr
Result: 40% reduction in promoter methylation (p<0.01)
Case 2: Agri-Biotech Firm
Challenge: Enhance drought resistance in wheat
Solution: 50μM seed pretreatment for 24hr
Result: 22% improvement in water retention capacity
5. Contact Our Experts
Email: info@vivalr.comPhone: (86) 15866781826
Available Mon-Fri 9:00-17:30 GMT+8
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