4-Nitrophenyl-beta-D-galactopyranoside CAS 3150-24-1

Chemical Name: 4-Nitrophenyl-beta-D-galactopyranoside
CAS No.: 3150-24-1
Molecular Fomula: C12H15NO8
Molecular weight: 301.25
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4-Nitrophenyl-beta-D-galactopyranoside (CAS 3150-24-1) Comprehensive User Guide

Contents

Product Specifications & Comparative Analysis

Parameter 4-Nitrophenyl-β-D-galactopyranoside Similar Substrates
CAS Number 3150-24-1 N/A
Purity ≥98% (HPLC) 90-95%
Solubility Water: 50 mg/mL 30-40 mg/mL
Storage Conditions -20°C protected from light Room temperature

Detailed Applications

Primary Use Cases:

  • Enzyme kinetics studies for β-galactosidase activity quantification
  • Diagnostic reagent component in clinical microbiology testing
  • Substrate in chromogenic assays for recombinant protein analysis

Industry-Specific Applications:

  • Pharmaceutical QC: Microbial limit testing
  • Biotech Research: Gene expression monitoring
  • Food Safety: Coliform detection systems

Optimized Usage Protocols

Standard Assay Protocol:

  1. Prepare 10 mM substrate solution in phosphate buffer (pH 6.8)
  2. Incubate with enzyme sample at 37°C for 15-30 minutes
  3. Measure absorbance at 405 nm using microplate reader

Client Case Study: BioResearch Labs Inc.

Application: High-throughput drug screening platform development

Challenge: Required stable substrate with minimal batch variability

Solution: Implemented our CGMP-grade substrate with lot-specific QC documentation

Result: 40% reduction in assay variability, 15% faster screening throughput

Client Case Study: PharmaDiagnostics Co., Ltd.

Application: Rapid diagnostic test kit manufacturing

Challenge: Needed cost-effective substrate meeting ISO 13485 standards

Solution: Custom-formulated bulk quantities with stability testing data

Result: Achieved 99.2% clinical sensitivity in final product validation

Request Technical Documentation & Pricing

Contact our biochemistry specialists for:

  • Custom formulation options
  • Bulk order discounts (50g+ quantities)
  • Technical support for assay development

Email: info@vivalr.com
Phone: (86) 15866781826

Related search terms: β-galactosidase substrate, chromogenic enzyme substrate CAS 3150-24-1, pNPG analog, diagnostic reagent chemicals, microbiology testing substrates.

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