4-Nitrophenyl alpha-D-galactopyranoside CAS 7493-95-0

Chemical Name: 4-Nitrophenyl alpha-D-galactopyranoside
CAS No.: 7493-95-0
Molecular Fomula: C12H15NO8
Molecular weight: 301.25
Appearance: Powder

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4-Nitrophenyl alpha-D-galactopyranoside (CAS 7493-95-0): Comprehensive User Guide

1. Product Overview & Specifications

CAS No.: 7493-95-0
Molecular Formula: C₁₂H₁₅NO₈
Molecular Weight: 301.25 g/mol
Appearance: White to off-white crystalline powder
Purity: ≥98% (HPLC)
Solubility: Soluble in water, DMSO, and methanol
Storage: -20°C, protected from light

Comparative Analysis

Parameter 4-Nitrophenyl α-D-galactopyranoside pNP β-D-glucoside pNP α-D-mannoside
Enzyme Specificity α-Galactosidase β-Glucosidase α-Mannosidase
Detection Sensitivity 0.1-5 U/mL 0.5-10 U/mL 1-15 U/mL
Reaction pH Range 4.5-7.5 5.0-8.0 6.0-7.5

2. Applications & Use Cases

Key Applications

  • α-Galactosidase enzyme activity quantification
  • Diagnostic kit development for Fabry disease
  • Quality control in biomanufacturing processes
  • Substrate specificity studies in glycosidase research

3. Step-by-Step Usage Protocols

Enzyme Activity Assay Protocol

  1. Prepare 10 mM substrate solution in 50 mM citrate buffer (pH 6.0)
  2. Mix 50 μL enzyme sample with 100 μL substrate solution
  3. Incubate at 37°C for 15-30 minutes
  4. Stop reaction with 50 μL 1M Na₂CO₃
  5. Measure absorbance at 405 nm

4. Customer Success Stories

Case Study 1: ABC Biotech

Challenge: Required high-purity substrate for GMP-compliant diagnostic kit production
Solution: Implemented batch-specific QC testing protocol
Result: Achieved 99.3% consistency across 15 production batches

Case Study 2: University of XYZ

Research Focus: Novel α-galactosidase inhibitors for gene therapy
Application: High-throughput screening of 5,000+ compounds
Outcome: Identified 3 lead candidates with IC₅₀ < 10 nM

5. Request a Quote or Sample

Contact our technical specialists for:
– Custom formulations
– Bulk pricing (100g+ quantities)
– MSDS & regulatory documentation

info@vivalr.com
(86) 15866781826

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