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Specialized in4-Nitrophenyl alpha-D-galactopyranoside CAS 7493-95-0and related product development and production

Chemical Name: 4-Nitrophenyl alpha-D-galactopyranoside
CAS No.: 7493-95-0
Molecular Fomula: C12H15NO8
Molecular weight: 301.25
Appearance: Powder

Samples: Available / Stock: Sufficient
Estimated delivery time: 3-5 days

4-Nitrophenyl alpha-D-galactopyranoside (CAS 7493-95-0): Comprehensive User Guide

Table of Contents

1. Product Overview & Specifications

CAS No.: 7493-95-0
Molecular Formula: C₁₂H₁₅NO₈
Molecular Weight: 301.25 g/mol
Appearance: White to off-white crystalline powder
Purity: ≥98% (HPLC)
Solubility: Soluble in water, DMSO, and methanol
Storage: -20°C, protected from light

Comparative Analysis

Parameter 4-Nitrophenyl α-D-galactopyranoside pNP β-D-glucoside pNP α-D-mannoside
Enzyme Specificity α-Galactosidase β-Glucosidase α-Mannosidase
Detection Sensitivity 0.1-5 U/mL 0.5-10 U/mL 1-15 U/mL
Reaction pH Range 4.5-7.5 5.0-8.0 6.0-7.5

2. Applications & Use Cases

Key Applications

  • α-Galactosidase enzyme activity quantification
  • Diagnostic kit development for Fabry disease
  • Quality control in biomanufacturing processes
  • Substrate specificity studies in glycosidase research

3. Step-by-Step Usage Protocols

Enzyme Activity Assay Protocol

  1. Prepare 10 mM substrate solution in 50 mM citrate buffer (pH 6.0)
  2. Mix 50 μL enzyme sample with 100 μL substrate solution
  3. Incubate at 37°C for 15-30 minutes
  4. Stop reaction with 50 μL 1M Na₂CO₃
  5. Measure absorbance at 405 nm

4. Customer Success Stories

Case Study 1: ABC Biotech

Challenge: Required high-purity substrate for GMP-compliant diagnostic kit production
Solution: Implemented batch-specific QC testing protocol
Result: Achieved 99.3% consistency across 15 production batches

Case Study 2: University of XYZ

Research Focus: Novel α-galactosidase inhibitors for gene therapy
Application: High-throughput screening of 5,000+ compounds
Outcome: Identified 3 lead candidates with IC₅₀ < 10 nM

5. Request a Quote or Sample

Contact our technical specialists for:
- Custom formulations
- Bulk pricing (100g+ quantities)
- MSDS & regulatory documentation

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Welcome to visit our factory . As the top chemical manufacturer, we have been striving for product quality, innovation , R&D, and customer service for the past 7 years. In the next 10 years or even longer, we are committed to becoming the most reliable chemical supplier in the world , creating a well-known international brand trusted by customers, with the spirit of “one meter wide, ten thousand meters deep”, we continue to focus on product research and development, continue to focus on customer service, continue to improve the supply chain service system, to create a professional chemical supply service team, to achieve win-win long-term cooperation. Please feel free to contact us if you have any questions.

Drug impurities to intermediates. Years of expertise.

Experience in the synthesis of complex compounds derived from American technology
Focus on the synthesis of difficult compounds
ISO 17034 dual system certification
National high-tech enterprise
5000+ square meters R&D center
A 70+ R&D team led by Chinese and foreign doctors
150 pieces (sets) of R&D equipment, 120+ fume hoods
18 patents

Solid QMS. Offer top-quality products and services.

Through more than ten years of continuous technological innovation and experience accumulation, the team's R&D personnel have established a complete quality management system for CATO, and have successively obtained ISO9001, ISO17034, CNAS and other international certifications.
It has successfully passed on-site inspections from the United States, Australia, Poland, Japan and domestic pharmaceutical company customers and institutions many times, providing high-quality one-stop services for R&D and production of new drugs around the world.

More than 150 sets of equipment to ensure quality.

The emphasis on quality is the basis for our survival and development, and is the most basic requirement for the products and services we provide. CATO has established a scientific, strict and comprehensive quality prevention and assurance system in accordance with international quality standards. Through advanced testing equipment such as GC-MS, chromatograph, nuclear magnetic resonance, scanning electron microscope, and ultraviolet light, quality management is implemented into every aspect of production and operation activities such as raw materials, inventory, production, testing, sales, verification, and training, and "All actions are controlled and traceable" as the work code of conduct for CATO employees.

Over 1000 cases. Synthesize complex compounds.

Currently, CATO serves more than 500 pharmaceutical companies, biotechnology and other companies around the world. Project types cover the entire cycle from pre-clinical to commercialization stages. As of 2020, CATO has delivered more than 1,000 projects in total.
The company has core technologies in key core technology fields such as high-end fluorine chemistry, asymmetric chiral synthesis, glycoside and biological enzyme technology, and continuous microreactors.

About the company

International Standard Chemicals Sell To All Over The World

FAQ

Does it support auditing?

Support, please make an appointment one day in advance. Company address: Room 716, Building 3, Ligao International Garden, No.1222 West Aoti Road, Lixia District, Jinan City, Shandong Province, P.R. China

Whether to provide samples?

Free samples can be provided for low value products, and small packaging specifications can be purchased for high value products.

Delivery time

Under normal circumstances, all items are in stock and will be shipped before 4 o'clock on the same day. Some products are produced to order, depending on the workshop situation and production schedule.

What is the production capacity?

At present, the company's workshop production equipment is mainly based on laboratories and pilot lines, with a maximum daily production capacity of 1.5 tons.

Can additional test indicators be added?

We can provide moisture, UV, IR, HNMR, CNMR, two-dimensional nuclear magnetic spectroscopy, optical rotation detection, elemental analysis, TGA analysis and residue analysis and other related tests to support personalized needs.

Technical direction

In the early stage, our company mainly engaged in drug impurity synthesis and standard product production, with core technologies in chirality, fluorine chemistry, and heterocycles.

Why Chem-iso’s delivery rate is guaranteed to be 99.99%

5000+

square meter R&D center

150+

China(set) of R&D equipment

150+

Fume hood

180+

R&D team

User’evaluation of us

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