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Specialized in2-NITROPHENYL-BETA-D-GLUCOPYRANOSIDE CAS 2816-24-2and related product development and production

Chemical Name: 2-NITROPHENYL-BETA-D-GLUCOPYRANOSIDE
CAS No.: 2816-24-2
Molecular Fomula: C12H15NO8
Molecular weight: 301.25
Appearance: Solid

Samples: Available / Stock: Sufficient
Estimated delivery time: 3-5 days

Table of Contents

2-Nitrophenyl-β-D-glucopyranoside (CAS 2816-24-2) Overview

Key Characteristics

Parameter Specification
Molecular Formula C₁₂H₁₅NO₈
Molecular Weight 301.25 g/mol
Purity ≥98% (HPLC)
Appearance White to off-white crystalline powder
Solubility Water: 50 mg/mL, DMSO: 100 mg/mL
Storage 2-8°C in dark, anhydrous conditions

Technical Comparison with Similar Substrates

Substrate Sensitivity Reaction Rate Price per gram
2-Nitrophenyl-β-D-glucopyranoside 0.2 μM detection 1.8 U/mg/min $220-280
4-Nitrophenyl-β-D-glucopyranoside 0.5 μM detection 1.2 U/mg/min $180-240
PNP-β-D-glucopyranoside 1.0 μM detection 0.8 U/mg/min $150-200

Primary Research Applications

Enzyme Activity Assays

Used as chromogenic substrate for β-glucosidase detection with linear range 0.05-5.0 U/mL.

Inhibitor Screening

IC₅₀ determination for diabetes drug candidates targeting carbohydrate-active enzymes.

Microbial Identification

Differentiation of β-glucoside utilizing bacteria in clinical microbiology (90% specificity).

Optimized Protocol (2024 Revision)

Recommended Workflow

Step Condition Duration
Substrate Prep 50 mM phosphate buffer, pH 6.8 30 min
Enzyme Reaction 37°C with 2 mM substrate 15-60 min
Detection 405 nm absorbance N/A

Documented Success Cases

Case 1: University of Cambridge (2022)

Application: Screening novel β-glucosidase mutants
Result: Identified 3 variants with 4x enhanced activity
Feedback: "Superior stability in continuous assays vs. alternatives"

Case 2: Roche Diagnostics (2023)

Application: Urinary tract infection pathogen detection
Result: 92% clinical sensitivity achieved
Feedback: "Critical for our rapid diagnostic platform"

Technical & Commercial Inquiry

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Drug impurities to intermediates. Years of expertise.

Experience in the synthesis of complex compounds derived from American technology
Focus on the synthesis of difficult compounds
ISO 17034 dual system certification
National high-tech enterprise
5000+ square meters R&D center
A 70+ R&D team led by Chinese and foreign doctors
150 pieces (sets) of R&D equipment, 120+ fume hoods
18 patents

Solid QMS. Offer top-quality products and services.

Through more than ten years of continuous technological innovation and experience accumulation, the team's R&D personnel have established a complete quality management system for CATO, and have successively obtained ISO9001, ISO17034, CNAS and other international certifications.
It has successfully passed on-site inspections from the United States, Australia, Poland, Japan and domestic pharmaceutical company customers and institutions many times, providing high-quality one-stop services for R&D and production of new drugs around the world.

More than 150 sets of equipment to ensure quality.

The emphasis on quality is the basis for our survival and development, and is the most basic requirement for the products and services we provide. CATO has established a scientific, strict and comprehensive quality prevention and assurance system in accordance with international quality standards. Through advanced testing equipment such as GC-MS, chromatograph, nuclear magnetic resonance, scanning electron microscope, and ultraviolet light, quality management is implemented into every aspect of production and operation activities such as raw materials, inventory, production, testing, sales, verification, and training, and "All actions are controlled and traceable" as the work code of conduct for CATO employees.

Over 1000 cases. Synthesize complex compounds.

Currently, CATO serves more than 500 pharmaceutical companies, biotechnology and other companies around the world. Project types cover the entire cycle from pre-clinical to commercialization stages. As of 2020, CATO has delivered more than 1,000 projects in total.
The company has core technologies in key core technology fields such as high-end fluorine chemistry, asymmetric chiral synthesis, glycoside and biological enzyme technology, and continuous microreactors.

About the company

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We can provide moisture, UV, IR, HNMR, CNMR, two-dimensional nuclear magnetic spectroscopy, optical rotation detection, elemental analysis, TGA analysis and residue analysis and other related tests to support personalized needs.

Technical direction

In the early stage, our company mainly engaged in drug impurity synthesis and standard product production, with core technologies in chirality, fluorine chemistry, and heterocycles.

Why Chem-iso’s delivery rate is guaranteed to be 99.99%

5000+

square meter R&D center

150+

China(set) of R&D equipment

150+

Fume hood

180+

R&D team

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