2-Nitrophenyl-beta-D-galactopyranoside (CAS 369-07-3): Comprehensive User Guide
Table of Contents
1. Product Overview & Specifications
CAS No.: 369-07-3
Molecular Formula: C12H15NO8
Molecular Weight: 301.25 g/mol
Appearance: White to off-white crystalline powder
Purity: ≥98% (HPLC)
Storage: 2-8°C in dark, dry conditions
Key Advantages:
- High sensitivity for β-galactosidase detection
- Superior solubility in aqueous buffers
- Linear detection range: 0.05-10 mM
2. Key Applications & Use Cases
2.1 Enzyme Activity Assays
Widely used as chromogenic substrate for:
- Quantifying β-galactosidase activity in E. coli cultures
- Monitoring gene expression in lacZ reporter systems
- Optimizing protein expression conditions
2.2 Diagnostic Applications
Essential component in:
- Microbial identification kits
- Point-of-care diagnostic devices
- Environmental monitoring systems
3. Performance Comparison
| Parameter | 2-Nitrophenyl-β-D-galactopyranoside | ONPG | X-Gal |
|---|---|---|---|
| Detection Sensitivity | 0.01 U/mL | 0.05 U/mL | Visual only |
| Absorbance Wavelength | 420 nm | 420 nm | N/A |
| Quantitative Capability | Yes | Yes | No |
4. Customer Success Stories
Case 1: BioTech Solutions Ltd. (Cambridge, UK)
Challenge: Required high-precision β-galactosidase detection for antibiotic development.
Solution: Implemented 369-07-3 in automated screening platform
Result: 40% faster lead compound identification
Case 2: Shanghai Diagnostics Co. (China)
Challenge: Needed stable substrate for portable diagnostic devices
Solution: Used 369-07-3 in lyophilized test kits
Result: 18-month shelf life achieved
5. Technical Support & Ordering
Contact our biochemistry experts:
✉️ info@vivalr.com
(86) 15866781826
Why Choose Us?
- ISO 9001-certified production
- Custom formulation services
- Global shipping capabilities
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