Hygromycin B (CAS 31282-04-9) User Guide & Application Insights 2025
Contents
- 1. Product Overview & Specifications
- 2. Key Applications & Use Cases
- 3. Usage Protocols & Best Practices
- 4. Industry Case Studies
- 5. Comparative Analysis with Alternatives
- 6. Technical Support & Purchasing Guidance
1. Product Overview & Specifications
Hygromycin B (CAS 31282-04-9) is an aminoglycoside antibiotic widely used in molecular biology for prokaryotic and eukaryotic cell selection.
| Parameter | Specification | Competitor A | Competitor B |
|---|---|---|---|
| Purity | ≥90% (HPLC) | 85-90% | 88-92% |
| Solubility | 50 mg/mL in H₂O | 40 mg/mL | 45 mg/mL |
| Working Concentration | 50-200 μg/mL | 100-250 μg/mL | 75-200 μg/mL |
2. Key Applications & Use Cases
Primary applications include:
- Selection marker in plasmid transfection
- Prokaryotic/eukaryotic antibiotic resistance screening
- Stable cell line development
- Gene knockout validation
3. Usage Protocols & Best Practices
Step-by-Step Protocol:
- Prepare stock solution at 50 mg/mL in sterile H₂O
- Filter-sterilize through 0.22 μm membrane
- Add to growth media at final concentration:
- Bacteria: 200 μg/mL
- Mammalian cells: 50-150 μg/mL
4. Industry Case Studies
Case Study 1: GenEdit Bio (San Francisco)
- Application: CRISPR-Cas9 stable cell line selection
- Result: 92% selection efficiency in HEK293 cells
- Dosage: 100 μg/mL for 14 days
Case Study 2: EuroPharma Research (Berlin)
- Application: Bacterial plasmid maintenance
- Result: 99% plasmid retention rate
- Dosage: 200 μg/mL in LB medium
5. Comparative Analysis with Alternatives
| Feature | Hygromycin B | Puromycin | Blasticidin |
|---|---|---|---|
| Selection Time | 5-7 days | 3-5 days | 7-10 days |
| Toxicity | Medium | High | Low |
6. Technical Support & Purchasing Guidance
For bulk orders or technical queries:
- Email: info@vivalr.com
- Phone: (86) 15866781826
- Request SDS documentation and purity certificates
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