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Specialized inHygromycin B CAS 31282-04-9and related product development and production

Chemical Name: Hygromycin B
CAS No.: 31282-04-9
Molecular Fomula: C20H37N3O13
Molecular weight: 527.52
Appearance: Powder

Samples: Available / Stock: Sufficient
Estimated delivery time: 3-5 days

Hygromycin B (CAS 31282-04-9) User Guide & Application Insights 2025

Contents

1. Product Overview & Specifications

Hygromycin B (CAS 31282-04-9) is an aminoglycoside antibiotic widely used in molecular biology for prokaryotic and eukaryotic cell selection.

Parameter Specification Competitor A Competitor B
Purity ≥90% (HPLC) 85-90% 88-92%
Solubility 50 mg/mL in H₂O 40 mg/mL 45 mg/mL
Working Concentration 50-200 μg/mL 100-250 μg/mL 75-200 μg/mL

2. Key Applications & Use Cases

Primary applications include:

  • Selection marker in plasmid transfection
  • Prokaryotic/eukaryotic antibiotic resistance screening
  • Stable cell line development
  • Gene knockout validation

3. Usage Protocols & Best Practices

Step-by-Step Protocol:

  1. Prepare stock solution at 50 mg/mL in sterile H₂O
  2. Filter-sterilize through 0.22 μm membrane
  3. Add to growth media at final concentration:
    • Bacteria: 200 μg/mL
    • Mammalian cells: 50-150 μg/mL

4. Industry Case Studies

Case Study 1: GenEdit Bio (San Francisco)

  • Application: CRISPR-Cas9 stable cell line selection
  • Result: 92% selection efficiency in HEK293 cells
  • Dosage: 100 μg/mL for 14 days

Case Study 2: EuroPharma Research (Berlin)

  • Application: Bacterial plasmid maintenance
  • Result: 99% plasmid retention rate
  • Dosage: 200 μg/mL in LB medium

5. Comparative Analysis with Alternatives

Feature Hygromycin B Puromycin Blasticidin
Selection Time 5-7 days 3-5 days 7-10 days
Toxicity Medium High Low

6. Technical Support & Purchasing Guidance

For bulk orders or technical queries:

  • Email: info@vivalr.com
  • Phone: (86) 15866781826
  • Request SDS documentation and purity certificates
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Drug impurities to intermediates. Years of expertise.

Experience in the synthesis of complex compounds derived from American technology
Focus on the synthesis of difficult compounds
ISO 17034 dual system certification
National high-tech enterprise
5000+ square meters R&D center
A 70+ R&D team led by Chinese and foreign doctors
150 pieces (sets) of R&D equipment, 120+ fume hoods
18 patents

Solid QMS. Offer top-quality products and services.

Through more than ten years of continuous technological innovation and experience accumulation, the team's R&D personnel have established a complete quality management system for CATO, and have successively obtained ISO9001, ISO17034, CNAS and other international certifications.
It has successfully passed on-site inspections from the United States, Australia, Poland, Japan and domestic pharmaceutical company customers and institutions many times, providing high-quality one-stop services for R&D and production of new drugs around the world.

More than 150 sets of equipment to ensure quality.

The emphasis on quality is the basis for our survival and development, and is the most basic requirement for the products and services we provide. CATO has established a scientific, strict and comprehensive quality prevention and assurance system in accordance with international quality standards. Through advanced testing equipment such as GC-MS, chromatograph, nuclear magnetic resonance, scanning electron microscope, and ultraviolet light, quality management is implemented into every aspect of production and operation activities such as raw materials, inventory, production, testing, sales, verification, and training, and "All actions are controlled and traceable" as the work code of conduct for CATO employees.

Over 1000 cases. Synthesize complex compounds.

Currently, CATO serves more than 500 pharmaceutical companies, biotechnology and other companies around the world. Project types cover the entire cycle from pre-clinical to commercialization stages. As of 2020, CATO has delivered more than 1,000 projects in total.
The company has core technologies in key core technology fields such as high-end fluorine chemistry, asymmetric chiral synthesis, glycoside and biological enzyme technology, and continuous microreactors.

About the company

International Standard Chemicals Sell To All Over The World

FAQ

Does it support auditing?

Support, please make an appointment one day in advance. Company address: Room 716, Building 3, Ligao International Garden, No.1222 West Aoti Road, Lixia District, Jinan City, Shandong Province, P.R. China

Whether to provide samples?

Free samples can be provided for low value products, and small packaging specifications can be purchased for high value products.

Delivery time

Under normal circumstances, all items are in stock and will be shipped before 4 o'clock on the same day. Some products are produced to order, depending on the workshop situation and production schedule.

What is the production capacity?

At present, the company's workshop production equipment is mainly based on laboratories and pilot lines, with a maximum daily production capacity of 1.5 tons.

Can additional test indicators be added?

We can provide moisture, UV, IR, HNMR, CNMR, two-dimensional nuclear magnetic spectroscopy, optical rotation detection, elemental analysis, TGA analysis and residue analysis and other related tests to support personalized needs.

Technical direction

In the early stage, our company mainly engaged in drug impurity synthesis and standard product production, with core technologies in chirality, fluorine chemistry, and heterocycles.

Why Chem-iso’s delivery rate is guaranteed to be 99.99%

5000+

square meter R&D center

150+

China(set) of R&D equipment

150+

Fume hood

180+

R&D team

User’evaluation of us

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