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Specialized in3,3′,5,5′-Tetramethylbenzidine Dihydrochlorideand related product development and production

Chemical Name: 3,3′,5,5′-Tetramethylbenzidine dihydrochloride
Synonyms: 4,4′-Diamino-3,3′,5,5′-tetramethylbiphenyl dihydrochloride
CAS.NO: 64285-73-0
Structural formula: C16H21ClN2
Molecular weight: 276.81
Appearance: White crystalline powder

Samples: Available / Stock: Sufficient
Estimated delivery time: 3-5 days

3,3',5,5'-Tetramethylbenzidine Dihydrochloride (TMB·2HCl) Comprehensive Guide

Table of Contents

Basic Specifications & Comparative Analysis

Parameter TMB Dihydrochloride Alternative Chromogens
CAS Number 64285-73-0 Varies by product
Molecular Formula C16H20N2·2HCl Depends on application
Purity ≥98% Typically 95-99%
Storage 2-8°C, dry, dark Room temperature (varies)

Applications & Functional Mechanisms

Core Applications

  • ELISA Diagnostics: Primary substrate for HRP-conjugated antibodies
  • Environmental Monitoring: Quantitative detection of residual chlorine, iron, and nitrites
  • Organic Contaminant Analysis: Benzo(a)pyrene detection at 0.1 μg/L sensitivity

Technical Advantages

  • Detection Range: 0.005 mg/L to 100 mg/L (analyte-dependent)
  • Linear Response: R2 >0.99 across pH 2.0-7.4
  • Colorimetric Stability: 24-hour signal retention post-reaction

Operational Protocols & Case Studies

Standard Workflow

  1. Prepare 10 mM TMB solution in deionized water
  2. Mix with peroxide buffer (1:1 ratio)
  3. Initiate enzymatic reaction (5-30 min, 25°C)
  4. Measure absorbance at 370/652 nm (kinetic) or 450 nm (stopped)

Case Example: Water Quality Monitoring

  • Client: Municipal Water Authority
  • Target: Simultaneous detection of Clres and Fe2+
  • Outcome: Achieved 0.005 mg/L Clres & 0.1 mg/L Fe2+ LOD

Client Success Stories

Biotech Manufacturer A

  • Application: High-throughput COVID-19 IgG ELISA
  • Volume: 50 kg annual consumption
  • Result: 99.8% batch consistency, 15% cost reduction

Environmental Lab B

  • Project: Industrial wastewater analysis
  • Solution: Custom TMB-based test kits
  • Impact: 40% faster analysis vs. traditional methods

Inquiry & Procurement Guidance

For technical specifications or bulk quotations:

Submit requirements:

  • Required purity (98-99.9%)
  • Packaging (1g-25kg scales)
  • Application-specific documentation needs
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The Manager has Something to Say

This is William, CEO of fousi chemical Chemical Co., Ltd.

Welcome to visit our factory . As the top chemical manufacturer, we have been striving for product quality, innovation , R&D, and customer service for the past 7 years. In the next 10 years or even longer, we are committed to becoming the most reliable chemical supplier in the world , creating a well-known international brand trusted by customers, with the spirit of “one meter wide, ten thousand meters deep”, we continue to focus on product research and development, continue to focus on customer service, continue to improve the supply chain service system, to create a professional chemical supply service team, to achieve win-win long-term cooperation. Please feel free to contact us if you have any questions.

Drug impurities to intermediates. Years of expertise.

Experience in the synthesis of complex compounds derived from American technology
Focus on the synthesis of difficult compounds
ISO 17034 dual system certification
National high-tech enterprise
5000+ square meters R&D center
A 70+ R&D team led by Chinese and foreign doctors
150 pieces (sets) of R&D equipment, 120+ fume hoods
18 patents

Solid QMS. Offer top-quality products and services.

Through more than ten years of continuous technological innovation and experience accumulation, the team's R&D personnel have established a complete quality management system for CATO, and have successively obtained ISO9001, ISO17034, CNAS and other international certifications.
It has successfully passed on-site inspections from the United States, Australia, Poland, Japan and domestic pharmaceutical company customers and institutions many times, providing high-quality one-stop services for R&D and production of new drugs around the world.

More than 150 sets of equipment to ensure quality.

The emphasis on quality is the basis for our survival and development, and is the most basic requirement for the products and services we provide. CATO has established a scientific, strict and comprehensive quality prevention and assurance system in accordance with international quality standards. Through advanced testing equipment such as GC-MS, chromatograph, nuclear magnetic resonance, scanning electron microscope, and ultraviolet light, quality management is implemented into every aspect of production and operation activities such as raw materials, inventory, production, testing, sales, verification, and training, and "All actions are controlled and traceable" as the work code of conduct for CATO employees.

Over 1000 cases. Synthesize complex compounds.

Currently, CATO serves more than 500 pharmaceutical companies, biotechnology and other companies around the world. Project types cover the entire cycle from pre-clinical to commercialization stages. As of 2020, CATO has delivered more than 1,000 projects in total.
The company has core technologies in key core technology fields such as high-end fluorine chemistry, asymmetric chiral synthesis, glycoside and biological enzyme technology, and continuous microreactors.

About the company

International Standard Chemicals Sell To All Over The World

FAQ

Does it support auditing?

Support, please make an appointment one day in advance. Company address: Room 716, Building 3, Ligao International Garden, No.1222 West Aoti Road, Lixia District, Jinan City, Shandong Province, P.R. China

Whether to provide samples?

Free samples can be provided for low value products, and small packaging specifications can be purchased for high value products.

Delivery time

Under normal circumstances, all items are in stock and will be shipped before 4 o'clock on the same day. Some products are produced to order, depending on the workshop situation and production schedule.

What is the production capacity?

At present, the company's workshop production equipment is mainly based on laboratories and pilot lines, with a maximum daily production capacity of 1.5 tons.

Can additional test indicators be added?

We can provide moisture, UV, IR, HNMR, CNMR, two-dimensional nuclear magnetic spectroscopy, optical rotation detection, elemental analysis, TGA analysis and residue analysis and other related tests to support personalized needs.

Technical direction

In the early stage, our company mainly engaged in drug impurity synthesis and standard product production, with core technologies in chirality, fluorine chemistry, and heterocycles.

Why Chem-iso’s delivery rate is guaranteed to be 99.99%

5000+

square meter R&D center

150+

China(set) of R&D equipment

150+

Fume hood

180+

R&D team

User’evaluation of us

"Chem-ios is my partner for years. Met at 2014 Shanghai CPHI Expo. Not interested then as had stable supplier. In Sep 2016, original supplier couldn't guarantee quality and delivery. Thought of Zhai and his company first. So far, have stable business relationship and good personal friendship."

Jasmine Hook

" In years of sourcing from China, first found working with Sincere Chemical great. They help in production, delivery and tech, saving time. When first asking Zhai for optical brightener quote, got detailed one with prices, trade details, company info, R&D and production capacity, packaging and raw material control."

Michael Cooper

" Hi, I'm Kim, Zhai's partner. Don't like him much (as he never gives more for my prices, haha), but still write customer feedback. Zhai's emails help me understand product and industry. Satisfied with his work ethic. He provides quotes and helps with work details, so I can focus on other things. He's the best supplier for me."

James Black

" Sincere Chemical team visited our USA factory in the year of 2017. We think highly of cooperation with them. We thanked you for the past excellent orders and best product quality. We also talked about some new products for future orders. We will be still in strategic cooperation in the future and help each other."

Lisa Goodwill

" I just got the product. The quality is awesome. I’m very happy. Hope to place soon a new order in your company. Also wish for our future business."

Ashton Spagg

"Sincere Chemical is reliable and professional. Its team is excellent in exporting and service, giving suggestions for our new project. Satisfied with service and product quality. Vendor code: 51465768."

Margo Brown

" I have visited Sincere Chemical company in the year 2018. Their company can be considered as one of the most professional, customer-focused, and product quality-driven companies that I have worked with in recent years."

Leo Rodgers

" We are a pharmaceutical manufacturer in Brazil. Happy to cooperate with Sincere Chemical, especially with Zhai. He is patient and friendly. Satisfied with his 24/7 service. We are not only cooperators but also best friends."

Milla Yoshi

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