3,3′,5,5′-Tetramethylbenzidine Dihydrochloride

3,3′,5,5′-Tetramethylbenzidine Dihydrochloride (TMB·2HCl) Comprehensive Guide

Table of Contents

• Basic Specifications & Comparative Analysis
• Applications & Functional Mechanisms
• Operational Protocols & Case Studies
• Client Success Stories
• Inquiry & Procurement Guidance

Basic Specifications & Comparative Analysis

Applications & Functional Mechanisms

Core Applications

• ELISA Diagnostics: Primary substrate for HRP-conjugated antibodies
• Environmental Monitoring: Quantitative detection of residual chlorine, iron, and nitrites
• Organic Contaminant Analysis: Benzo(a)pyrene detection at 0.1 μg/L sensitivity

Technical Advantages

• Detection Range: 0.005 mg/L to 100 mg/L (analyte-dependent)
• Linear Response: R² >0.99 across pH 2.0-7.4
• Colorimetric Stability: 24-hour signal retention post-reaction

Operational Protocols & Case Studies

Standard Workflow

1. Prepare 10 mM TMB solution in deionized water
2. Mix with peroxide buffer (1:1 ratio)
3. Initiate enzymatic reaction (5-30 min, 25°C)
4. Measure absorbance at 370/652 nm (kinetic) or 450 nm (stopped)

Case Example: Water Quality Monitoring

• Client: Municipal Water Authority
• Target: Simultaneous detection of Cl_res and Fe²⁺
• Outcome: Achieved 0.005 mg/L Cl_res & 0.1 mg/L Fe²⁺ LOD

Client Success Stories

Biotech Manufacturer A

• Application: High-throughput COVID-19 IgG ELISA
• Volume: 50 kg annual consumption
• Result: 99.8% batch consistency, 15% cost reduction

Environmental Lab B

• Project: Industrial wastewater analysis
• Solution: Custom TMB-based test kits
• Impact: 40% faster analysis vs. traditional methods

Inquiry & Procurement Guidance

For technical specifications or bulk quotations:

• Email: info@vivalr.com
• Tel: (86) 15866781826

Submit requirements:

• Required purity (98-99.9%)
• Packaging (1g-25kg scales)
• Application-specific documentation needs