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Specialized inPUROMYCIN DIHYDROCHLORIDE CAS 58-58-2and related product development and production

Chemical Name: PUROMYCIN DIHYDROCHLORIDE
CAS No.: 58-58-2
Molecular Fomula: C22H30ClN7O5
Molecular weight: 507.98
Appearance: White to off-white Solid

Samples: Available / Stock: Sufficient
Estimated delivery time: 3-5 days

Table of Contents

Puromycin Dihydrochloride CAS 58-58-2: Technical Specifications

Key Parameters

Parameter Specification
CAS Number 58-58-2
Molecular Formula C22H29ClN7O5
Purity ≥98% (HPLC)
Solubility 50 mg/mL in Water
Storage -20°C, desiccated

Comparative Analysis

Feature Puromycin G418 Hygromycin B
Selection Mechanism Protein synthesis inhibitor Ribosomal interference Translation blocker
Effective Concentration 1-10 µg/mL 50-1000 µg/mL 50-200 µg/mL
Action Speed 24-48 hours 3-7 days 5-10 days

Research Applications

Core Functions

  • Stable cell line selection (eukaryotic systems)
  • Translation termination in pulse-chase experiments
  • Antibiotic selection in CRISPR-edited cells

Experimental Scenarios

  • Mammalian cell transfection validation
  • Ribosome profiling studies
  • Inducible expression system optimization

Experimental Protocol

Standard Workflow

  1. Prepare 10 mg/mL stock solution in sterile PBS
  2. Filter-sterilize using 0.22 µm membrane
  3. Apply 2-5 µg/mL final concentration to culture medium
  4. Monitor cell viability for 72 hours

Critical Notes

  • Light-sensitive: protect from prolonged exposure
  • Effective pH range: 6.0-7.5
  • Not suitable for plant cell systems

Documented Applications

Case 1: Stable CHO Cell Line Development

Biopharma Inc. achieved 95% transfection efficiency using 3 µg/mL puromycin selection over 10-day period for monoclonal antibody production.

Case 2: Viral Vector Packaging Optimization

Gene Therapy Labs reduced selection timeline from 14 to 7 days through pulsed puromycin treatment (5 µg/mL, 48h intervals).

Client Implementation Records

University Research Team

  • Institution: Stanford Cell Biology Department
  • Application: CRISPR-Cas9 edited HEK293T cell selection
  • Result: 98% pure population in 96 hours

Biotech Startup

  • Company: NeoBiotechnologies
  • Application: High-throughput drug screening models
  • Result: Reduced false positives by 40% vs. alternative antibiotics

Technical Support & Ordering

For protocol optimization or bulk purchasing:

  • Email: info@vivalr.com
  • Phone: (86) 15866781826

Why Choose Our Product:

  • Batch-to-batch consistency (HPLC certified)
  • Custom concentration formulations available
  • GMP-grade production options
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This is William, CEO of fousi chemical Chemical Co., Ltd.

Welcome to visit our factory . As the top chemical manufacturer, we have been striving for product quality, innovation , R&D, and customer service for the past 7 years. In the next 10 years or even longer, we are committed to becoming the most reliable chemical supplier in the world , creating a well-known international brand trusted by customers, with the spirit of “one meter wide, ten thousand meters deep”, we continue to focus on product research and development, continue to focus on customer service, continue to improve the supply chain service system, to create a professional chemical supply service team, to achieve win-win long-term cooperation. Please feel free to contact us if you have any questions.

Drug impurities to intermediates. Years of expertise.

Experience in the synthesis of complex compounds derived from American technology
Focus on the synthesis of difficult compounds
ISO 17034 dual system certification
National high-tech enterprise
5000+ square meters R&D center
A 70+ R&D team led by Chinese and foreign doctors
150 pieces (sets) of R&D equipment, 120+ fume hoods
18 patents

Solid QMS. Offer top-quality products and services.

Through more than ten years of continuous technological innovation and experience accumulation, the team's R&D personnel have established a complete quality management system for CATO, and have successively obtained ISO9001, ISO17034, CNAS and other international certifications.
It has successfully passed on-site inspections from the United States, Australia, Poland, Japan and domestic pharmaceutical company customers and institutions many times, providing high-quality one-stop services for R&D and production of new drugs around the world.

More than 150 sets of equipment to ensure quality.

The emphasis on quality is the basis for our survival and development, and is the most basic requirement for the products and services we provide. CATO has established a scientific, strict and comprehensive quality prevention and assurance system in accordance with international quality standards. Through advanced testing equipment such as GC-MS, chromatograph, nuclear magnetic resonance, scanning electron microscope, and ultraviolet light, quality management is implemented into every aspect of production and operation activities such as raw materials, inventory, production, testing, sales, verification, and training, and "All actions are controlled and traceable" as the work code of conduct for CATO employees.

Over 1000 cases. Synthesize complex compounds.

Currently, CATO serves more than 500 pharmaceutical companies, biotechnology and other companies around the world. Project types cover the entire cycle from pre-clinical to commercialization stages. As of 2020, CATO has delivered more than 1,000 projects in total.
The company has core technologies in key core technology fields such as high-end fluorine chemistry, asymmetric chiral synthesis, glycoside and biological enzyme technology, and continuous microreactors.

About the company

International Standard Chemicals Sell To All Over The World

FAQ

Does it support auditing?

Support, please make an appointment one day in advance. Company address: Room 716, Building 3, Ligao International Garden, No.1222 West Aoti Road, Lixia District, Jinan City, Shandong Province, P.R. China

Whether to provide samples?

Free samples can be provided for low value products, and small packaging specifications can be purchased for high value products.

Delivery time

Under normal circumstances, all items are in stock and will be shipped before 4 o'clock on the same day. Some products are produced to order, depending on the workshop situation and production schedule.

What is the production capacity?

At present, the company's workshop production equipment is mainly based on laboratories and pilot lines, with a maximum daily production capacity of 1.5 tons.

Can additional test indicators be added?

We can provide moisture, UV, IR, HNMR, CNMR, two-dimensional nuclear magnetic spectroscopy, optical rotation detection, elemental analysis, TGA analysis and residue analysis and other related tests to support personalized needs.

Technical direction

In the early stage, our company mainly engaged in drug impurity synthesis and standard product production, with core technologies in chirality, fluorine chemistry, and heterocycles.

Why Chem-iso’s delivery rate is guaranteed to be 99.99%

5000+

square meter R&D center

150+

China(set) of R&D equipment

150+

Fume hood

180+

R&D team

User’evaluation of us

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